A novel transducible chimeric phage from Escherichia coli O157

H7 Sakai strain encoding Stx1 production

Domonkos Sváb, Balázs Bálint, Gergely Maróti, I. Tóth

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Shiga toxin-producing Escherichia coli (STEC), and especially enterohaemorrhagic E. coli (EHEC) are important, highly virulent zoonotic and food-borne pathogens. The genes encoding their key virulence factors, the Shiga toxins, are distributed by converting bacteriophages, the Stx phages. In this study we isolated a new type of inducible Stx phage carrying the stx1 gene cluster from the prototypic EHEC O157:H7 Sakai strain. The phage showed Podoviridae morphology, and was capable of converting the E. coli K-12 MG1655 strain to Shiga toxin-producing phenotype. The majority of the phage genes originate from the stx2-encoding Sakai prophage Sp5, with major rearrangements in its genome. Beside certain minor recombinations, the genomic region originally containing the stx2 genes in Sp5 was replaced by a region containing six open reading frames from prophage Sp15 including stx1 genes. The rearranged genome, together with the carriage of stx1 genes, the morphology and the capability of lysogenic conversion represent a new type of recombinant Stx1 converting phage from the Sakai strain.

Original languageEnglish
Pages (from-to)42-47
Number of pages6
JournalInfection, Genetics and Evolution
Volume29
DOIs
Publication statusPublished - Jan 1 2015

Fingerprint

Escherichia coli O157
bacteriophages
Bacteriophages
gene
toxin
Enterohemorrhagic Escherichia coli
Prophages
enterohemorrhagic Escherichia coli
Genes
genes
Podoviridae
genome
Genome
Shiga Toxins
Shiga Toxin
Escherichia coli K12
Shiga-Toxigenic Escherichia coli
Shiga toxin
verotoxins
Shiga toxin-producing Escherichia coli

Keywords

  • Lysogenic conversion
  • O157:H7
  • Phage recombination
  • Sakai strain
  • Stx phages

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Genetics
  • Molecular Biology
  • Microbiology
  • Infectious Diseases
  • Microbiology (medical)
  • Medicine(all)

Cite this

A novel transducible chimeric phage from Escherichia coli O157 : H7 Sakai strain encoding Stx1 production. / Sváb, Domonkos; Bálint, Balázs; Maróti, Gergely; Tóth, I.

In: Infection, Genetics and Evolution, Vol. 29, 01.01.2015, p. 42-47.

Research output: Contribution to journalArticle

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