A novel thermophilic methane-oxidising γ-Proteobacterium

Levente Bodrossy, K. Kovács, Ian R. McDonald, J. Colin Murrell

Research output: Contribution to journalArticle

71 Citations (Scopus)

Abstract

A novel thermophilic methane-oxidising bacterium was isolated from underground hot springs in Hungary. Strain HB grew on methane at up to 72°C, the highest recorded growth temperature for a methanotroph. 16S rDNA phylogenetic analysis showed that strain HB was the first known representative of a novel, deep-branching group of the γ-Proteobacteria quite distinct from extant methanotrophs. The nucleotide sequence of pmoA, encoding particulate methane monooxygenase, was determined for this novel thermophile. Sequence comparison showed that the methane monooxygenase of this strain was most closely related to that of Methylocaldum and Methylococcus species. Particulate methane monooxygenase gene fragments having a high degree of identity to that of pmoA from strain HB were amplified by PCR from DNA isolated from thermophilic methane-oxidising enrichments inoculated with hot spring samples (55-70°C) from Japan, suggesting that this novel genus, for which we informally suggest the name 'Methylothermus', is widespread in thermophilic environments. Copyright (C) 1999 Federation of European Microbiological Societies.

Original languageEnglish
Pages (from-to)335-341
Number of pages7
JournalFEMS Microbiology Letters
Volume170
Issue number2
DOIs
Publication statusPublished - Jan 15 1999

Fingerprint

methane monooxygenase
Proteobacteria
Methane
Hot Springs
Methylococcus
Methylococcaceae
Hungary
Ribosomal DNA
Names
Japan
Polymerase Chain Reaction
Temperature
DNA
Growth
Genes

Keywords

  • 'Methylothermus'
  • 16S rRNA phylogeny
  • Methanotroph
  • Particulate methane monooxygenase gene
  • Thermophile

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology
  • Applied Microbiology and Biotechnology
  • Microbiology

Cite this

A novel thermophilic methane-oxidising γ-Proteobacterium. / Bodrossy, Levente; Kovács, K.; McDonald, Ian R.; Murrell, J. Colin.

In: FEMS Microbiology Letters, Vol. 170, No. 2, 15.01.1999, p. 335-341.

Research output: Contribution to journalArticle

Bodrossy, Levente ; Kovács, K. ; McDonald, Ian R. ; Murrell, J. Colin. / A novel thermophilic methane-oxidising γ-Proteobacterium. In: FEMS Microbiology Letters. 1999 ; Vol. 170, No. 2. pp. 335-341.
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