A novel target for the promotion of dermal wound healing: Ryanodine receptors

Döníz Degovics, P. Hartmann, István Balázs Németh, Noémi Árva-Nagy, Enikő Kaszonyi, Edit Szél, Gerda Strifler, Balázs Bende, L. Krenács, L. Kemény, Gábor Erős

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Ryanodine receptors have an important role in the regulation of intracellular calcium levels in the nervous system and muscle. It has been described that ryanodine receptors influence keratinocyte differentiation and barrier homeostasis. Our goal was to examine the role of ryanodine receptors in the healing of full-thickness dermal wounds by means of in vitro and in vivo methods. The effect of ryanodine receptors on wound healing, microcirculation and inflammation was assessed in an in vivo mouse wound healing model, using skin fold chambers in the dorsal region, and in HaCaT cell scratch wound assay in vitro. SKH-1 mice were subjected to sterile saline (n = 36) or ryanodine receptor agonist 4-chloro-m-cresol (0.5 mM) (n = 42) or ryanodine receptor antagonist dantrolene (100 μM) (n = 42). Application of ryanodine receptor agonist 4-chloro-m-cresol did not influence the studied parameters significantly, whereas ryanodine receptor antagonist dantrolene accelerated the wound closure. Inhibition of the calcium channel also increased the vessel diameters in the wound edges during the process of healing and increased the blood flow in the capillaries at all times of measurement. Furthermore, application of dantrolene decreased xanthine-oxidoreductase activity during the inflammatory phase of wound healing. Inhibition of ryanodine receptor-mediated effects positively influence wound healing. Thus, dantrolene may be of therapeutic potential in the treatment of wounds.

Original languageEnglish
Pages (from-to)17-24
Number of pages8
JournalToxicology and Applied Pharmacology
Volume366
DOIs
Publication statusPublished - Mar 1 2019

Fingerprint

Ryanodine Receptor Calcium Release Channel
Wound Healing
Skin
Dantrolene
Wounds and Injuries
Xanthine Dehydrogenase
Microcirculation
Neurology
Calcium Channels
Keratinocytes
Nervous System
Muscle
Assays
Homeostasis
Blood
Inflammation
Calcium
Muscles

Keywords

  • Calcium channel
  • Ryanodine receptor
  • Skin fold chamber
  • Wound healing

ASJC Scopus subject areas

  • Toxicology
  • Pharmacology

Cite this

A novel target for the promotion of dermal wound healing : Ryanodine receptors. / Degovics, Döníz; Hartmann, P.; Németh, István Balázs; Árva-Nagy, Noémi; Kaszonyi, Enikő; Szél, Edit; Strifler, Gerda; Bende, Balázs; Krenács, L.; Kemény, L.; Erős, Gábor.

In: Toxicology and Applied Pharmacology, Vol. 366, 01.03.2019, p. 17-24.

Research output: Contribution to journalArticle

Degovics, D, Hartmann, P, Németh, IB, Árva-Nagy, N, Kaszonyi, E, Szél, E, Strifler, G, Bende, B, Krenács, L, Kemény, L & Erős, G 2019, 'A novel target for the promotion of dermal wound healing: Ryanodine receptors', Toxicology and Applied Pharmacology, vol. 366, pp. 17-24. https://doi.org/10.1016/j.taap.2019.01.021
Degovics, Döníz ; Hartmann, P. ; Németh, István Balázs ; Árva-Nagy, Noémi ; Kaszonyi, Enikő ; Szél, Edit ; Strifler, Gerda ; Bende, Balázs ; Krenács, L. ; Kemény, L. ; Erős, Gábor. / A novel target for the promotion of dermal wound healing : Ryanodine receptors. In: Toxicology and Applied Pharmacology. 2019 ; Vol. 366. pp. 17-24.
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