A novel SPECT-based approach reveals early mechanisms of central and peripheral inflammation after cerebral ischemia

Krisztián Szigeti, I. Horváth, Dániel S. Veres, Bernadett Martinecz, Nikolett Lénárt, Noémi Kovács, Erika Bakcsa, Alexa Márta, Mariann Semjéni, Domokos Máthé, Ádám Dénes

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Inflammation that develops in the brain and peripheral organs after stroke contributes profoundly to poor outcome of patients. However, mechanisms through which inflammation impacts on brain injury and overall outcome are improperly understood, in part because the earliest inflammatory events after brain injury are not revealed by current imaging tools. Here, we show that single-photon emission computed tomography (NanoSPECT/CT Plus) allows visualization of blood brain barrier (BBB) injury after experimental stroke well before changes can be detected with magnetic resonance imaging (MRI). Early 99mTc-DTPA (diethylene triamine pentaacetic acid) signal changes predict infarct development and systemic inflammation preceding experimental stroke leads to very early perfusion deficits and increased BBB injury within 2 hours after the onset of ischemia. Acute brain injury also leads to peripheral inflammation and immunosuppression, which contribute to poor outcome of stroke patients. The SPECT imaging revealed early (within 2 hours) changes in perfusion, barrier function and inflammation in the lungs and the gut after experimental stroke, with good predictive value for the development of histopathologic changes at later time points. Collectively, visualization of early inflammatory changes after stroke could open new translational research avenues to elucidate the interactions between central and peripheral inflammation and to evaluate in vivo 'multi-system' effects of putative anti-inflammatory treatments.

Original languageEnglish
Pages (from-to)1921-1929
Number of pages9
JournalJournal of Cerebral Blood Flow and Metabolism
Volume35
Issue number12
DOIs
Publication statusPublished - Dec 1 2015

Fingerprint

Single-Photon Emission-Computed Tomography
Brain Ischemia
Brain Injuries
Stroke
Inflammation
Blood-Brain Barrier
Perfusion
Pentetic Acid
Translational Medical Research
Immunosuppression
Pneumonia
Anti-Inflammatory Agents
Ischemia
Magnetic Resonance Imaging
Acids
Brain

Keywords

  • BBB; brain injury; cerebral ischemia; SPECT imaging; systemic inflammation

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine
  • Clinical Neurology
  • Neurology

Cite this

A novel SPECT-based approach reveals early mechanisms of central and peripheral inflammation after cerebral ischemia. / Szigeti, Krisztián; Horváth, I.; Veres, Dániel S.; Martinecz, Bernadett; Lénárt, Nikolett; Kovács, Noémi; Bakcsa, Erika; Márta, Alexa; Semjéni, Mariann; Máthé, Domokos; Dénes, Ádám.

In: Journal of Cerebral Blood Flow and Metabolism, Vol. 35, No. 12, 01.12.2015, p. 1921-1929.

Research output: Contribution to journalArticle

Szigeti, K, Horváth, I, Veres, DS, Martinecz, B, Lénárt, N, Kovács, N, Bakcsa, E, Márta, A, Semjéni, M, Máthé, D & Dénes, Á 2015, 'A novel SPECT-based approach reveals early mechanisms of central and peripheral inflammation after cerebral ischemia', Journal of Cerebral Blood Flow and Metabolism, vol. 35, no. 12, pp. 1921-1929. https://doi.org/10.1038/jcbfm.2015.174
Szigeti, Krisztián ; Horváth, I. ; Veres, Dániel S. ; Martinecz, Bernadett ; Lénárt, Nikolett ; Kovács, Noémi ; Bakcsa, Erika ; Márta, Alexa ; Semjéni, Mariann ; Máthé, Domokos ; Dénes, Ádám. / A novel SPECT-based approach reveals early mechanisms of central and peripheral inflammation after cerebral ischemia. In: Journal of Cerebral Blood Flow and Metabolism. 2015 ; Vol. 35, No. 12. pp. 1921-1929.
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