A novel RNA-binding peptide regulates the establishment of the Medicago truncatula-Sinorhizobium meliloti nitrogen-fixing symbiosis

Philippe Laporte, Béatrice Satiat-Jeunemaître, Isabel Velasco, Tibor Csorba, Willem Van De Velde, Anna Campalans, J. Burgyán, Miguel Arevalo-Rodriguez, Martin Crespi

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Plants use a variety of small peptides for cell to cell communication during growth and development. Leguminous plants are characterized by their ability to develop nitrogen-fixing nodules via an interaction with symbiotic bacteria. During nodule organogenesis, several so-called nodulin genes are induced, including large families that encode small peptides. Using a three-hybrid approach in yeast cells, we identified two new small nodulins, MtSNARP1 and MtSNARP2 (for small nodulin acidic RNA-binding protein), which interact with the RNA of MtENOD40, an early induced nodulin gene showing conserved RNA secondary structures. The SNARPs are acidic peptides showing single-stranded RNA-binding activity in vitro and are encoded by a small gene family in Medicago truncatula. These peptides exhibit two new conserved motifs and a putative signal peptide that redirects a GFP fusion to the endoplasmic reticulum both in protoplasts and during symbiosis, suggesting they are secreted. MtSNARP2 is expressed in the differentiating region of the nodule together with several early nodulin genes. MtSNARP2 RNA interference (RNAi) transgenic roots showed aberrant early senescent nodules where differentiated bacteroids degenerate rapidly. Hence, a functional symbiotic interaction may be regulated by secreted RNA-binding peptides.

Original languageEnglish
Pages (from-to)24-38
Number of pages15
JournalPlant Journal
Volume62
Issue number1
DOIs
Publication statusPublished - Apr 2010

Fingerprint

Medicago truncatula
nodulins
Sinorhizobium meliloti
Ensifer meliloti
Symbiosis
symbiosis
Nitrogen
peptides
RNA
Peptides
nitrogen
Genes
genes
cell communication
RNA-binding proteins
RNA-Binding Proteins
Organogenesis
Protoplasts
Protein Sorting Signals
RNA Interference

Keywords

  • Cell-to-cell communication
  • Functional symbiosome
  • Medicago truncatula
  • RNA-binding protein
  • Secreted peptide

ASJC Scopus subject areas

  • Plant Science
  • Cell Biology
  • Genetics

Cite this

Laporte, P., Satiat-Jeunemaître, B., Velasco, I., Csorba, T., Van De Velde, W., Campalans, A., ... Crespi, M. (2010). A novel RNA-binding peptide regulates the establishment of the Medicago truncatula-Sinorhizobium meliloti nitrogen-fixing symbiosis. Plant Journal, 62(1), 24-38. https://doi.org/10.1111/j.1365-313X.2009.04121.x

A novel RNA-binding peptide regulates the establishment of the Medicago truncatula-Sinorhizobium meliloti nitrogen-fixing symbiosis. / Laporte, Philippe; Satiat-Jeunemaître, Béatrice; Velasco, Isabel; Csorba, Tibor; Van De Velde, Willem; Campalans, Anna; Burgyán, J.; Arevalo-Rodriguez, Miguel; Crespi, Martin.

In: Plant Journal, Vol. 62, No. 1, 04.2010, p. 24-38.

Research output: Contribution to journalArticle

Laporte, P, Satiat-Jeunemaître, B, Velasco, I, Csorba, T, Van De Velde, W, Campalans, A, Burgyán, J, Arevalo-Rodriguez, M & Crespi, M 2010, 'A novel RNA-binding peptide regulates the establishment of the Medicago truncatula-Sinorhizobium meliloti nitrogen-fixing symbiosis', Plant Journal, vol. 62, no. 1, pp. 24-38. https://doi.org/10.1111/j.1365-313X.2009.04121.x
Laporte, Philippe ; Satiat-Jeunemaître, Béatrice ; Velasco, Isabel ; Csorba, Tibor ; Van De Velde, Willem ; Campalans, Anna ; Burgyán, J. ; Arevalo-Rodriguez, Miguel ; Crespi, Martin. / A novel RNA-binding peptide regulates the establishment of the Medicago truncatula-Sinorhizobium meliloti nitrogen-fixing symbiosis. In: Plant Journal. 2010 ; Vol. 62, No. 1. pp. 24-38.
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