A novel pleiotropic effect of aspirin: Beneficial regulation of pro- and anti-inflammatory mechanisms in microglial cells

Diana Kata, I. Földesi, Liliana Z. Feher, Laszlo Hackler, L. Puskás, K. Gulya

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Aspirin, one of the most widely used non-steroidal anti-inflammatory drugs, has extensively studied effects on the cardiovascular system. To reveal further pleiotropic, beneficial effects of aspirin on a number of pro- and anti-inflammatory microglial mechanisms, we performed morphometric and functional studies relating to phagocytosis, pro- and anti-inflammatory cytokine production (IL-1β, tumor necrosis factor-α (TNF-α) and IL-10, respectively) and analyzed the expression of a number of inflammation-related genes, including those related to the above functions, in pure microglial cells. We examined the effects of aspirin (0.1 mM and 1 mM) in unchallenged (control) and bacterial lipopolysaccharide (LPS)-challenged secondary microglial cultures. Aspirin affected microglial morphology and functions in a dose-dependent manner as it inhibited LPS-elicited microglial activation by promoting ramification and the inhibition of phagocytosis in both concentrations. Remarkably, aspirin strongly reduced the pro-inflammatory IL-1β and TNF-α production, while it increased the anti-inflammatory IL-10 level in LPS-challenged cells. Moreover, aspirin differentially regulated the expression of a number of inflammation-related genes as it downregulated such pro-inflammatory genes as Nos2, Kng1, IL1β, Ptgs2 or Ccr1, while it upregulated some anti-inflammatory genes such as IL10, Csf2, Cxcl1, Ccl5 or Tgfb1. Thus, the use of aspirin could be beneficial for the prophylaxis of certain neurodegenerative disorders as it effectively ameliorates inflammation in the brain.

Original languageEnglish
Pages (from-to)61-74
Number of pages14
JournalBrain Research Bulletin
Volume132
DOIs
Publication statusPublished - Jun 1 2017

Fingerprint

Aspirin
Anti-Inflammatory Agents
Interleukin-10
Lipopolysaccharides
Interleukin-1
Phagocytosis
Genes
Tumor Necrosis Factor-alpha
Inflammation
Encephalitis
Cardiovascular System
Neurodegenerative Diseases
Down-Regulation
Cytokines
Pharmaceutical Preparations

Keywords

  • Anti-inflammation
  • Aspirin
  • Gene expression
  • Lipopolysaccharide
  • Phagocytosis
  • Pro-inflammation

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

A novel pleiotropic effect of aspirin : Beneficial regulation of pro- and anti-inflammatory mechanisms in microglial cells. / Kata, Diana; Földesi, I.; Feher, Liliana Z.; Hackler, Laszlo; Puskás, L.; Gulya, K.

In: Brain Research Bulletin, Vol. 132, 01.06.2017, p. 61-74.

Research output: Contribution to journalArticle

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