A novel phenylalanine ammonia-lyase from Kangiella koreensis

Andrea Varga, Zsófia Bata, Pál Csuka, Diana Monica Bordea, B. Vértessy, Adriana Marcovici, Florin Dan Irimie, L. Poppe, László Csaba Bencze

Research output: Contribution to journalArticle

Abstract

This study describes the cloning of the gene encoding a novel phenylalanine ammonia-lyase from Kangiella koreensis (KkPAL) into pET19b expression vector. Optimization of protein expression and purification conditions yielded 15 mg pure soluble protein from one liter of E. coli culture. Enzymatic activity measurements of the ammonia elimination reaction from different natural aromatic amino acids proved the protein to be a phenylalanine ammonia-lyase. The isolated protein showed remarkably high, 81.7 °C melting temperature, making it especially suitable for biocatalytic applications.

Original languageEnglish
Pages (from-to)293-308
Number of pages16
JournalStudia Universitatis Babes-Bolyai Chemia
Volume62
Issue number3
DOIs
Publication statusPublished - Jan 1 2017

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Phenylalanine Ammonia-Lyase
Proteins
Aromatic Amino Acids
Gene encoding
Cloning
Ammonia
Escherichia coli
Purification
Melting point

Keywords

  • Kangiella koreensis
  • Optimization
  • Phenylalanine ammonia-lyase
  • Protein expression

ASJC Scopus subject areas

  • Chemistry(all)

Cite this

Varga, A., Bata, Z., Csuka, P., Bordea, D. M., Vértessy, B., Marcovici, A., ... Bencze, L. C. (2017). A novel phenylalanine ammonia-lyase from Kangiella koreensis. Studia Universitatis Babes-Bolyai Chemia, 62(3), 293-308. https://doi.org/10.24193/subbchem.2017.3.25

A novel phenylalanine ammonia-lyase from Kangiella koreensis. / Varga, Andrea; Bata, Zsófia; Csuka, Pál; Bordea, Diana Monica; Vértessy, B.; Marcovici, Adriana; Irimie, Florin Dan; Poppe, L.; Bencze, László Csaba.

In: Studia Universitatis Babes-Bolyai Chemia, Vol. 62, No. 3, 01.01.2017, p. 293-308.

Research output: Contribution to journalArticle

Varga, A, Bata, Z, Csuka, P, Bordea, DM, Vértessy, B, Marcovici, A, Irimie, FD, Poppe, L & Bencze, LC 2017, 'A novel phenylalanine ammonia-lyase from Kangiella koreensis', Studia Universitatis Babes-Bolyai Chemia, vol. 62, no. 3, pp. 293-308. https://doi.org/10.24193/subbchem.2017.3.25
Varga, Andrea ; Bata, Zsófia ; Csuka, Pál ; Bordea, Diana Monica ; Vértessy, B. ; Marcovici, Adriana ; Irimie, Florin Dan ; Poppe, L. ; Bencze, László Csaba. / A novel phenylalanine ammonia-lyase from Kangiella koreensis. In: Studia Universitatis Babes-Bolyai Chemia. 2017 ; Vol. 62, No. 3. pp. 293-308.
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