A novel phenylalanine ammonia-lyase from Kangiella koreensis

Andrea Varga, Zsófia Bata, Pál Csuka, Diana Monica Bordea, Beáta G. Vértessy, Adriana Marcovici, Florin Dan Irimie, László Poppe, László Csaba Bencze

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

This study describes the cloning of the gene encoding a novel phenylalanine ammonia-lyase from Kangiella koreensis (KkPAL) into pET19b expression vector. Optimization of protein expression and purification conditions yielded 15 mg pure soluble protein from one liter of E. coli culture. Enzymatic activity measurements of the ammonia elimination reaction from different natural aromatic amino acids proved the protein to be a phenylalanine ammonia-lyase. The isolated protein showed remarkably high, 81.7 °C melting temperature, making it especially suitable for biocatalytic applications.

Original languageEnglish
Pages (from-to)293-308
Number of pages16
JournalStudia Universitatis Babes-Bolyai Chemia
Volume62
Issue number3
DOIs
Publication statusPublished - Jan 1 2017

Keywords

  • Kangiella koreensis
  • Optimization
  • Phenylalanine ammonia-lyase
  • Protein expression

ASJC Scopus subject areas

  • Chemistry(all)

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  • Cite this

    Varga, A., Bata, Z., Csuka, P., Bordea, D. M., Vértessy, B. G., Marcovici, A., Irimie, F. D., Poppe, L., & Bencze, L. C. (2017). A novel phenylalanine ammonia-lyase from Kangiella koreensis. Studia Universitatis Babes-Bolyai Chemia, 62(3), 293-308. https://doi.org/10.24193/subbchem.2017.3.25