A novel method for the isolation of carboxypeptidase B.

E. Dala, A. Kiss, P. Südi, B. Szajáni

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Carboxypeptidase B was isolated from porcine pancreas by selective heat treatment of the autolyzed tissue at 60 degrees C and pH 6.0 the presence of phosphate ions. The heat treatment was followed by ammonium sulfate fractionation and ion-exchange in a batch system. The method could also be used for the isolation of carboxypeptidase B from beef pancreas.

Original languageEnglish
Pages (from-to)327-333
Number of pages7
JournalActa Biochimica et Biophysica Hungarica
Volume21
Issue number4
Publication statusPublished - 1986

Fingerprint

Carboxypeptidase B
Pancreas
Hot Temperature
Heat treatment
Beef
Ion Exchange
Ammonium Sulfate
Fractionation
Ion exchange
Swine
Phosphates
Ions
Tissue

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics

Cite this

A novel method for the isolation of carboxypeptidase B. / Dala, E.; Kiss, A.; Südi, P.; Szajáni, B.

In: Acta Biochimica et Biophysica Hungarica, Vol. 21, No. 4, 1986, p. 327-333.

Research output: Contribution to journalArticle

Dala, E. ; Kiss, A. ; Südi, P. ; Szajáni, B. / A novel method for the isolation of carboxypeptidase B. In: Acta Biochimica et Biophysica Hungarica. 1986 ; Vol. 21, No. 4. pp. 327-333.
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