Carboxypeptidase B was isolated from porcine pancreas by selective heat treatment of the autolyzed tissue at 60 degrees C and pH 6.0 the presence of phosphate ions. The heat treatment was followed by ammonium sulfate fractionation and ion-exchange in a batch system. The method could also be used for the isolation of carboxypeptidase B from beef pancreas.
|Number of pages||7|
|Journal||Acta biochimica et biophysica Hungarica|
|Publication status||Published - Dec 1 1986|
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