A novel method for the isolation of carboxypeptidase B.

E. Dala, A. Kiss, P. Südi, B. Szajáni

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Carboxypeptidase B was isolated from porcine pancreas by selective heat treatment of the autolyzed tissue at 60 degrees C and pH 6.0 the presence of phosphate ions. The heat treatment was followed by ammonium sulfate fractionation and ion-exchange in a batch system. The method could also be used for the isolation of carboxypeptidase B from beef pancreas.

Original languageEnglish
Pages (from-to)327-333
Number of pages7
JournalActa biochimica et biophysica Hungarica
Volume21
Issue number4
Publication statusPublished - Dec 1 1986

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry

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