A novel method for the identification of factors involved in host-pathogen interactions in Drosophila melanogaster

Beáta Kari, János Zsámboki, Viktor Honti, Gábor Csordás, Róbert Márkus, István Andó, Éva Kurucz

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

A new method was established, standardized and validated for screening factors involved in the response to septic injury in Drosophila melanogaster. The method, based on inducing lesion by removing the tarsal segments of the first pair of legs of Drosophila adults and exposing them to different bacteria, imitates injury that often occurs in the natural habitat. The method is easy to perform, highly reproducible and suitable for large-scale genetic screens with the aim of identifying factors involved in host-pathogen interactions. The technique was validated by using mutant variations of different components of the immune response, blood clotting as well as the involvement of a number of genes known to be instrumental in the humoral and cell-mediated immune responses of Drosophila was confirmed. Moreover, the combination of the present method with antibiotic treatment allows the screening of potential antimicrobial drugs in vivo.

Original languageEnglish
Pages (from-to)76-82
Number of pages7
JournalJournal of Immunological Methods
Volume398-399
Issue number1
DOIs
Publication statusPublished - Oct 7 2013

Keywords

  • Drosophila model
  • Immune defense
  • Screen
  • Septic injury
  • Virulence
  • Wound healing

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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