A novel method for isolation of lipoxygenase-1 isoenzyme from soybean meal

Á Sz Németh, B. Szajáni, J. Sz Márczy, M. L. Simon

Research output: Contribution to journalArticle

Abstract

A novel method has been elaborated for the isolation of lipoxygenase-1 (Lox-1) isoenzyme from soybean meal. The essential step of the procedure is the selective heat treatment of soybean meal extract at 70°C in a medium of 0.05 ionic strength and pH 5.2 for 5 min. The heat treated extract practically free from lipoxygenase-2 (Lox-2) and lipoxygenase-3 (Lox-3) isoenzymes was purified by DEAE-cellulose ion exchange chromatography. The resulted lipoxygenase-1 isoenzyme was homogeneous as judged by PAGE. A tenfold purification and a relatively high yield (39%) was achieved.

Original languageEnglish
Pages (from-to)35-38
Number of pages4
JournalHungarian Journal of Industrial Chemistry
Volume29
Issue number1
Publication statusPublished - 2001

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Isoenzymes
Lipoxygenase
DEAE-Cellulose
Chromatography
Ionic strength
Purification
Cellulose
Ion exchange
Heat treatment

Keywords

  • Glycine max
  • Lipoxygenase isoenzymes
  • Selective heat treatment
  • Soybean

ASJC Scopus subject areas

  • Chemistry (miscellaneous)
  • Chemistry(all)
  • Chemical Engineering(all)

Cite this

A novel method for isolation of lipoxygenase-1 isoenzyme from soybean meal. / Németh, Á Sz; Szajáni, B.; Márczy, J. Sz; Simon, M. L.

In: Hungarian Journal of Industrial Chemistry, Vol. 29, No. 1, 2001, p. 35-38.

Research output: Contribution to journalArticle

Németh, Á Sz ; Szajáni, B. ; Márczy, J. Sz ; Simon, M. L. / A novel method for isolation of lipoxygenase-1 isoenzyme from soybean meal. In: Hungarian Journal of Industrial Chemistry. 2001 ; Vol. 29, No. 1. pp. 35-38.
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