A novel method for increasing the frequency of somatic embryogenesis in wheat tissue culture by NaCl and KCl supplementation

G. Galiba, Y. Yamada

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

The effect of NaCl, KCl and LiCl on the growth and morphogeneis of tissue cultures originating from immature embryos of four wheat (Triticum aestivum L.) and one triticale (Triticosecale)varieties was investigated. The morphogenetic pathway to plant regeneration in Chinese Spring wheat was determined as incomplete somatic embryogenesis because the differentiation and subsequent germination of the shoot apices happened in the early phase of embryo development. Culture medium supplemented by NaCl suppressed the differentiation of shoot apices resulting in the development of more typical somatic embryoids. Forty mM concentrations of both NaCl or KCl increased the formation of somatic embryos in Chinese Spring. Arthur and GK Kincso wheat varieties while Lasko triticale regenerated well without the addition. The salts inhibited plantlet formation from somatic embryoids so the salts supplement should be omitted. Forty mM LiCl inhibited growth while 10mM LiCl had no effect on growth or embryogenesis.

Original languageEnglish
Pages (from-to)55-58
Number of pages4
JournalPlant Cell Reports
Volume7
Issue number1
DOIs
Publication statusPublished - Jan 1988

Fingerprint

Triticosecale
somatic embryogenesis
tissue culture
embryogenesis
salts
wheat
shoots
spring wheat
somatic embryos
embryo (plant)
plantlets
Triticum aestivum
culture media
immatures
germination
methodology

ASJC Scopus subject areas

  • Plant Science

Cite this

A novel method for increasing the frequency of somatic embryogenesis in wheat tissue culture by NaCl and KCl supplementation. / Galiba, G.; Yamada, Y.

In: Plant Cell Reports, Vol. 7, No. 1, 01.1988, p. 55-58.

Research output: Contribution to journalArticle

@article{57cba29c30f844a3b71757d65221105e,
title = "A novel method for increasing the frequency of somatic embryogenesis in wheat tissue culture by NaCl and KCl supplementation",
abstract = "The effect of NaCl, KCl and LiCl on the growth and morphogeneis of tissue cultures originating from immature embryos of four wheat (Triticum aestivum L.) and one triticale (Triticosecale)varieties was investigated. The morphogenetic pathway to plant regeneration in Chinese Spring wheat was determined as incomplete somatic embryogenesis because the differentiation and subsequent germination of the shoot apices happened in the early phase of embryo development. Culture medium supplemented by NaCl suppressed the differentiation of shoot apices resulting in the development of more typical somatic embryoids. Forty mM concentrations of both NaCl or KCl increased the formation of somatic embryos in Chinese Spring. Arthur and GK Kincso wheat varieties while Lasko triticale regenerated well without the addition. The salts inhibited plantlet formation from somatic embryoids so the salts supplement should be omitted. Forty mM LiCl inhibited growth while 10mM LiCl had no effect on growth or embryogenesis.",
author = "G. Galiba and Y. Yamada",
year = "1988",
month = "1",
doi = "10.1007/BF00272978",
language = "English",
volume = "7",
pages = "55--58",
journal = "Plant Cell Reports",
issn = "0721-7714",
publisher = "Springer Verlag",
number = "1",

}

TY - JOUR

T1 - A novel method for increasing the frequency of somatic embryogenesis in wheat tissue culture by NaCl and KCl supplementation

AU - Galiba, G.

AU - Yamada, Y.

PY - 1988/1

Y1 - 1988/1

N2 - The effect of NaCl, KCl and LiCl on the growth and morphogeneis of tissue cultures originating from immature embryos of four wheat (Triticum aestivum L.) and one triticale (Triticosecale)varieties was investigated. The morphogenetic pathway to plant regeneration in Chinese Spring wheat was determined as incomplete somatic embryogenesis because the differentiation and subsequent germination of the shoot apices happened in the early phase of embryo development. Culture medium supplemented by NaCl suppressed the differentiation of shoot apices resulting in the development of more typical somatic embryoids. Forty mM concentrations of both NaCl or KCl increased the formation of somatic embryos in Chinese Spring. Arthur and GK Kincso wheat varieties while Lasko triticale regenerated well without the addition. The salts inhibited plantlet formation from somatic embryoids so the salts supplement should be omitted. Forty mM LiCl inhibited growth while 10mM LiCl had no effect on growth or embryogenesis.

AB - The effect of NaCl, KCl and LiCl on the growth and morphogeneis of tissue cultures originating from immature embryos of four wheat (Triticum aestivum L.) and one triticale (Triticosecale)varieties was investigated. The morphogenetic pathway to plant regeneration in Chinese Spring wheat was determined as incomplete somatic embryogenesis because the differentiation and subsequent germination of the shoot apices happened in the early phase of embryo development. Culture medium supplemented by NaCl suppressed the differentiation of shoot apices resulting in the development of more typical somatic embryoids. Forty mM concentrations of both NaCl or KCl increased the formation of somatic embryos in Chinese Spring. Arthur and GK Kincso wheat varieties while Lasko triticale regenerated well without the addition. The salts inhibited plantlet formation from somatic embryoids so the salts supplement should be omitted. Forty mM LiCl inhibited growth while 10mM LiCl had no effect on growth or embryogenesis.

UR - http://www.scopus.com/inward/record.url?scp=0003130102&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0003130102&partnerID=8YFLogxK

U2 - 10.1007/BF00272978

DO - 10.1007/BF00272978

M3 - Article

C2 - 24241416

AN - SCOPUS:0003130102

VL - 7

SP - 55

EP - 58

JO - Plant Cell Reports

JF - Plant Cell Reports

SN - 0721-7714

IS - 1

ER -