A novel labeling procedure of anteiso-fatty acid-containing lipids in staphylococci for investigating the effect of penicillin on lipid release

Ferenc Rozgonyi, Helmut Hahn, Harald Labischinski, Peter Giesbrecht

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

l-[4,5-3H]isoleucine was introduced to label anteiso-fatty acid (AIFA)-containing lipids in Staphylococcus aureus SG 511. After an overnight incubation in peptone broth in the presence of 37 kBq l-[4,5-3H]isoleucine/ml, 8.5-13% of the total radioactivity applied was found to be incorporated into the cells. 22.4-25.6% of the incorporated radioactivity was found in AIFA-containing lipids extracted by chloroform-methanol-water (2:1:02, v/v/v) at pH 2. The interphase contained 70-75% of the incorporated radioactivity. Lipoteichoic acid, extracted by phenol-water 80:20, w/v) contained less than 1% of the incorporated radioactivity, as measured after purification by hydrophobic interaction chromoatography on octyl sepharose gel. Within 1 h after addition of 10μg/ml penicillin G to exponentially growing cultures of S. aureus, that led to non-lytic death of the cells, 11.9-18.1% of the incorporated l-[4,5-3H] isoleucine label were released. Lipids containing AIFA were excreted to 5.4-8.4% of total incorporated activity; this amount represents more than 1/4 of the labeled cellular lipids.

Original languageEnglish
Pages (from-to)11-15
Number of pages5
JournalFEMS microbiology letters
Volume71
Issue number1-2
DOIs
Publication statusPublished - Sep 1 1990

Keywords

  • Anteiso-fatty acid
  • Isoleucine label
  • LTA
  • Lipid release
  • Novel labeling
  • Penicillin action
  • Staphylococcus

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology
  • Genetics

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