A novel hydrogel scaffold for periodontal ligament stem cells

Krisztina Nagy, Orsolya LÁNg, JÚLia LÁNg, Katalin Perczel-KovÁCh, Szabolcs Gyulai-GaÁL, KristÓF Kádár, LÁSzlÓ Kohidai, GÁBor Varga

Research output: Contribution to journalArticle

2 Citations (Scopus)


Periodontal ligament stem cells (PDLSCs) possess extensive regeneration potential. However, their therapeutic application demands ascaffold with appropriate properties. HydroMatrix (HydM) is a novel injectable peptide nanofiber hydrogel developed recently for cell culture. Ouraim was to test whether HydM would be a suitable scaffold for proliferation and osteogenic differentiation of PDLSCs. PDLSCs were seeded on noncoated or HydM-coated surfaces. Both real-time impedance analysis and cell viability assay documented cell growth on HydM. PDLSCs showedhealthy, fibroblast-like morphology on the hydrogel. After a 3-week-long culture in osteogenic medium, mineralization was much more intense inHydM cultures compared to control. Alkaline phosphatase activity of the cells grown on the gels reached the non-coated control levels. Our dataprovided evidence that PDLSCs can adhere, survive, migrate, and proliferate on HydM and this gel also supports their osteogenic differentiation. Wefirst applied impedimetry for dental stem cells cultured on a scaffold. HydM is ideal for in vitro studies of PDLSCs. It may also serve not only as areference material but also in the future as a promising biocompatible scaffold for preclinical studies.

Original languageEnglish
Pages (from-to)162-170
Number of pages9
JournalInterventional Medicine and Applied Science
Issue number3
Publication statusPublished - Sep 2018


  • Cell proliferation
  • HydroMatrix
  • Impedimetry
  • Osteogenic differentiation
  • Periodontal ligament
  • Stem cell

ASJC Scopus subject areas

  • Medicine(all)

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