A novel fruitfly protein under developmental control degrades uracil-DNA

Angéla Békési, Mária Pukáncsik, Villo Muha, Imre Zagyva, Ibolya Leveles, E. Hunyadi-Gulyás, E. Klement, Katalin F. Medzihradszky, Z. Kele, A. Erdei, Ferenc Felföldi, Emese Kónya, B. Vértessy

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Uracil in DNA may arise by cytosine deamination or thymine replacement and is removed during DNA repair. Fruitfly larvae lack two repair enzymes, the major uracil-DNA glycosylase and dUTPase, and may accumulate uracil-DNA. We asked if larval tissues contain proteins that specifically recognize uracil-DNA. We show that the best hit of pull-down on uracil-DNA is the protein product of the Drosophila melanogaster gene CG18410. This protein binds to both uracil-DNA and normal DNA but degrades only uracil-DNA; it is termed Uracil-DNA Degrading Factor (UDE). The protein has detectable homology only to a group of sequences present in genomes of pupating insects. It is under detection level in the embryo, most of the larval stages and in the imago, but is strongly upregulated right before pupation. In Schneider 2 cells, UDE mRNA is upregulated by ecdysone. UDE represents a new class of proteins that process uracil-DNA with potential involvement in metamorphosis.

Original languageEnglish
Pages (from-to)643-648
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume355
Issue number3
DOIs
Publication statusPublished - Apr 13 2007

Fingerprint

Uracil
DNA
Proteins
Insect Genome
Uracil-DNA Glycosidase
Repair
Genes
Ecdysone
Drosophila Proteins
Deamination
Thymine
Cytosine
DNA Repair
Larva
Embryonic Structures

Keywords

  • Development
  • Drosophila melanogaster
  • Metamorphosis
  • Nuclease
  • Pupating insect
  • Uracil-DNA

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

A novel fruitfly protein under developmental control degrades uracil-DNA. / Békési, Angéla; Pukáncsik, Mária; Muha, Villo; Zagyva, Imre; Leveles, Ibolya; Hunyadi-Gulyás, E.; Klement, E.; Medzihradszky, Katalin F.; Kele, Z.; Erdei, A.; Felföldi, Ferenc; Kónya, Emese; Vértessy, B.

In: Biochemical and Biophysical Research Communications, Vol. 355, No. 3, 13.04.2007, p. 643-648.

Research output: Contribution to journalArticle

Békési, Angéla ; Pukáncsik, Mária ; Muha, Villo ; Zagyva, Imre ; Leveles, Ibolya ; Hunyadi-Gulyás, E. ; Klement, E. ; Medzihradszky, Katalin F. ; Kele, Z. ; Erdei, A. ; Felföldi, Ferenc ; Kónya, Emese ; Vértessy, B. / A novel fruitfly protein under developmental control degrades uracil-DNA. In: Biochemical and Biophysical Research Communications. 2007 ; Vol. 355, No. 3. pp. 643-648.
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