A Novel Effect of the New Antileukemic Drug, 2-Chloro-2′-deoxyadenosine, in Human Lymphocytes

M. Sasvariszekely, Z. Piroth, Z. Kazimierczuk, M. Staub

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

2-chloro-2′-deoxryadenosine (Cl-dAdo) is a potent drug against hairy cell leukemia and other lymphomas. Its effects on the metabolism of labeled deoxycytidine (5-[3H]dCyd) and deoxythymidine ([3H]dThd) were investigated in short term cultures of human lymphocytes. In the presence of Cl-dAdo, a more pronounced inhibition of DNA synthesis was found, accompanied with a much higher accumulation of radioactivity in dTTP than in dCTP. This difference disappeared in the presence of exogenous dThd. Analysis of intracellular metabolites of 5-[3H]dCyd showed an increase in [3H]dCTP used for liponucleotide biosynthesis and a decrease in [3H]dUMP formation in the presence of Cl-dAdo, suggesting the possible inhibition of dCMP deaminase.

Original languageEnglish
Pages (from-to)1378-1384
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume203
Issue number3
DOIs
Publication statusPublished - Sep 30 1994

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Lymphocytes
Hairy Cell Leukemia
Deoxycytidine
Biosynthesis
Radioactivity
Metabolites
Cell culture
Metabolism
Pharmaceutical Preparations
Thymidine
Lymphoma
DNA
2'-deoxycytidine 5'-triphosphate
2'-deoxyadenosine
thymidine 5'-triphosphate
2'-deoxyuridylic acid

ASJC Scopus subject areas

  • Molecular Biology
  • Biophysics
  • Biochemistry

Cite this

A Novel Effect of the New Antileukemic Drug, 2-Chloro-2′-deoxyadenosine, in Human Lymphocytes. / Sasvariszekely, M.; Piroth, Z.; Kazimierczuk, Z.; Staub, M.

In: Biochemical and Biophysical Research Communications, Vol. 203, No. 3, 30.09.1994, p. 1378-1384.

Research output: Contribution to journalArticle

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