A novel, complement-mediated way to enhance the interplay between macrophages, dendritic cells and T lymphocytes

Noémi Sándor, Domonkos Pap, József Prechl, Anna Erdei, Zsuzsa Bajtay

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Recently it has been reported that human C3-deficiency is associated with impairments in dendritic cell differentiation. Here we investigated how complement C3 influences the phenotype and functional activity of human dendritic cells. We show that human monocyte-derived dendritic cells (MDCs) when incubated with native, hemolytically active C3, bind the activation fragments of C3 covalently. This reaction directs MDCs to increase expression of MHCII, CD83 and CD86, moreover it results in a significantly enhanced secretion of TNF-α, IL-6 and IL-8. A further functional consequence of C3b-fixation is the elevated capacity of the dendritic cells to stimulate allogeneic T cells. The distinct role of covalently fixed C3-fragments is strongly supported by our results obtained with MDCs where CD11b expression was downregulated by siRNA. To reveal the possible in vivo significance of the present findings we modelled a phenomenon occurring during inflammation, where C3 is produced locally by activated macrophages. In these cocultures MDCs were found to fix substantial amounts of macrophage derived C3-fragments on their cell membrane. Our data provide compelling evidence that antigen presenting cells arising in complement-sufficient environment mature to competent stimulators of T cells.

Original languageEnglish
Pages (from-to)438-448
Number of pages11
JournalMolecular Immunology
Volume47
Issue number2-3
DOIs
Publication statusPublished - Dec 1 2009

Keywords

  • Complement C3
  • Dendritic cell
  • Macrophages
  • T cell activation

ASJC Scopus subject areas

  • Immunology
  • Molecular Biology

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