A novel aspect of NADPH production in ageing Penicillium chrysogenum

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NADPH is involved in many basically important anabolic processes. For a long time, pentose phosphate pathway (PPS) was regarded as the most important source of NADPH in fungi. Here we present evidence of a metabolic switch to an alternative NADPH-producing pathway in ageing Penicillium chrysogenum cultures, which involves NADP+-specific isocitrate dehydrogenase (NADP +-ID) rather than PPS enzymes. Considering the main biochemical functions of NADPH, we propose that NADP+-ID could have deep impact on many physiological processes switched on glucose deprivation including proteinase production or penicillin biosynthesis. We also demonstrate that although the alternative pathway was inferior to PPS when the fungus was grown on well-utilisable carbon sources yet it could have an important role in fatty acid biosynthesis as well as in the maintenance of high intracellular NADPH/NADP+ ratios.

Original languageEnglish
Pages (from-to)115-121
Number of pages7
JournalActa biologica Hungarica
Issue number1
Publication statusPublished - Jul 21 2006



  • Ageing
  • NADP-specific isocitrate dehydrogenase
  • NADPH production
  • Penicillium chrysogenum

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Environmental Science(all)
  • Neurology

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