A novel and rapid prediction assay for the effectiveness of IL-6 receptor specific antisense oligonucleotides by proliferation inhibition of an interleukin-6 dependent cell line

Valeria Lia Varga, Andras Falus, Eva Pallinger, István Novák, Kristóf A. Fülöp, Sara Toth, Stefan Rose-John

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Interleukin-6 (IL-6) belongs to a family of cytokines that use receptors consisting of a common signal-transducing chain (gp130). Baf/3 cells transfected with the human IL-6 receptor (IL-6R) and gp130 (Baf/3-gp130/IL-6R) can only grow in medium containing IL-6. We attempted to interrupt the signal transducing pathway of IL-6 with the help of antisense oligonucleotides (ASOs) designed against the IL-6R. We used 18 different kinds of antisense oligonucleotides of overlapping sequences around the translational start codon of the human IL-6R. Sense ASOs were used as a control. The proliferation of cells was analysed by H-thymidine incorporation. Cell surface expression of the IL-6R was assessed by FACS analysis. We identified three ASOs which strongly inhibited the proliferation of IL-6 dependent transfected Baf/3 cells. Flow cytometric studies on the suppression of surface expression of IL-6R by ASOs showed a similar pattern. These results should help to clarify the structural requirements of functionally effective ASOs in the inhibition of IL-6R.

Original languageEnglish
Pages (from-to)253-256
Number of pages4
JournalCell biology international
Volume25
Issue number3
DOIs
Publication statusPublished - Jan 1 2001

Keywords

  • Antisense
  • Interleukin-6 receptor
  • Proliferation

ASJC Scopus subject areas

  • Cell Biology

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