A novel aldose/aldehyde reductase protects transgenic plants against lipid peroxidation under chemical and drought stresses

Attila Oberschall, Mária Deák, Katalin Török, L. Sass, I. Vass, Izabella Kovács, A. Fehér, D. Dudits, G. Horváth

Research output: Contribution to journalArticle

181 Citations (Scopus)

Abstract

Rapid accumulation of toxic products from reactions of reactive oxygen species (ROS) with lipids and proteins significantly contributes to the damage of crop plants under biotic and abiotic stresses. Here we have identified a stress-activated alfalfa gene encoding a novel plant NADPH-dependent aldose/aldehyde reductase that also exhibited characteristics of the homologous human enzyme. The recombinant alfalfa enzyme is active on 4-hydroxynon-2-enal, a known cytotoxic lipid peroxide degradation product. Ectopic synthesis of this enzyme in transgenic tobacco plants provided considerable tolerance against oxidative damage caused by paraquat and heavy metal treatment. These transformants could also resist a long period of water deficiency and exhibited improved recovery after rehydration. We found a reduced production of lipid peroxidation-derived reactive aldehydes in these transformed plants under different stresses. These studies reveal a new and efficient detoxification pathway in plants.

Original languageEnglish
Pages (from-to)437-446
Number of pages10
JournalPlant Journal
Volume24
Issue number4
DOIs
Publication statusPublished - 2000

Fingerprint

aldoses
aldehyde reductase
Aldehyde Reductase
Genetically Modified Plants
Droughts
Lipid Peroxidation
transgenic plants
lipid peroxidation
water stress
Medicago sativa
alfalfa
Enzymes
enzymes
Paraquat
paraquat
Lipid Peroxides
Fluid Therapy
Poisons
rehydration
lipids

Keywords

  • Aldo-keto reductase
  • Detoxification
  • Drought tolerance
  • Lipid peroxidation
  • Stress resistance

ASJC Scopus subject areas

  • Plant Science

Cite this

A novel aldose/aldehyde reductase protects transgenic plants against lipid peroxidation under chemical and drought stresses. / Oberschall, Attila; Deák, Mária; Török, Katalin; Sass, L.; Vass, I.; Kovács, Izabella; Fehér, A.; Dudits, D.; Horváth, G.

In: Plant Journal, Vol. 24, No. 4, 2000, p. 437-446.

Research output: Contribution to journalArticle

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AU - Deák, Mária

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AU - Sass, L.

AU - Vass, I.

AU - Kovács, Izabella

AU - Fehér, A.

AU - Dudits, D.

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