A novel adjustable automated system for inducing chronic intermittent hypoxia in mice

Dora Polšek, Marcel Bago, Marija Živaljiæ, Ivana Rosenzweig, Z. Lacza, Sreæko Gajoviæ

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Background Sleep apnea is a chronic, widely underdiagnosed condition characterized by disruption of sleep architecture and intermittent hypoxia due to short cessations of breathing. It is a major independent risk factor for myocardial infarction, congestive heart failure and stroke as well as one of the rare modifiable risk factors for Alzheimer's Dementia. Reliable animal disease models are needed to understand the link between sleep apnea and the various clinically linked disorders. New method An automated system for inducing hypoxia was developed, in which the major improvement was the possibility to efficiently adjust the length and intensity of hypoxia in two different periods. The chamber used a small volume of gas allowing for fast exchanges of different oxygen levels. The mice were kept in their cages adapted with the system on the cage lid. As a proof of principle, they were exposed to a three week period of intermittent hypoxia for 8 hours a day, with 90 s intervals of 5, 7% and 21% oxygen to validate the model. Treated (n = 8) and control mice (no hypoxia, n = 7) were handled in the same manner and their hippocampal brain regions compared by histology. Results The chamber provided a fast, reliable and precise intermittent hypoxia, without inducing noticeable side effects to the animals. The validation experiment showed that apoptotic neurons in the hippocampus were more numerous in the mice exposed to intermittent hypoxia than in the control group, in all tested hippocampal regions (cornu ammonis 1 (CA1) P <0.001; cornu ammonis 3 (CA3) P <0.001; and dentate gyrus (DG) P = 0.023). In both, control and hypoxic conditions, there was a significantly higher number of apoptotic neurons in the DG compared to the CA1 and CA3 subfields (P <0.001).Conclusion The new design of a hypoxic chamber provides a fast, adjustable and reliable model of obstructive sleep apnea, which was validated by apoptosis of hippocampal neurons.

Original languageEnglish
Article numbere0174896
JournalPLoS One
Volume12
Issue number3
DOIs
Publication statusPublished - Mar 1 2017

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hypoxia
Neurons
Hippocampus
sleep apnea
mice
Animals
Oxygen
neurons
Dentate Gyrus
Sleep Apnea Syndromes
Histology
cages
risk factors
animal disease models
Brain
Gases
oxygen
Animal Disease Models
Apoptosis
lids

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Polšek, D., Bago, M., Živaljiæ, M., Rosenzweig, I., Lacza, Z., & Gajoviæ, S. (2017). A novel adjustable automated system for inducing chronic intermittent hypoxia in mice. PLoS One, 12(3), [e0174896]. https://doi.org/10.1371/journal.pone.0174896

A novel adjustable automated system for inducing chronic intermittent hypoxia in mice. / Polšek, Dora; Bago, Marcel; Živaljiæ, Marija; Rosenzweig, Ivana; Lacza, Z.; Gajoviæ, Sreæko.

In: PLoS One, Vol. 12, No. 3, e0174896, 01.03.2017.

Research output: Contribution to journalArticle

Polšek, D, Bago, M, Živaljiæ, M, Rosenzweig, I, Lacza, Z & Gajoviæ, S 2017, 'A novel adjustable automated system for inducing chronic intermittent hypoxia in mice', PLoS One, vol. 12, no. 3, e0174896. https://doi.org/10.1371/journal.pone.0174896
Polšek, Dora ; Bago, Marcel ; Živaljiæ, Marija ; Rosenzweig, Ivana ; Lacza, Z. ; Gajoviæ, Sreæko. / A novel adjustable automated system for inducing chronic intermittent hypoxia in mice. In: PLoS One. 2017 ; Vol. 12, No. 3.
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