A novel α-amylase inhibitor from amaranth (Amaranthus hypocondriacus) seeds

Alicia Chagolla-Lopez, Alejandro Blanco-Labra, A. Pátthy, Roberto Sánchez, Sándor Pongor

Research output: Contribution to journalArticle

79 Citations (Scopus)

Abstract

The major α-amylase inhibitor (AAI) present in the seeds of Amaranthus hypocondriacus, a variety of the Mexican crop plant amaranth, is a 32- residue-long polypeptide with three disulfide bridges. Purified AAI strongly inhibits the α-amylase activity of insect larvae (Tribolium castaneum and Prostephanus truncatus) and does not inhibit proteases and mammalian α- amylases. AAI was sequenced with the automated Edman method, and the disulfide bridges were localized using enzymatic and chemical fragmentation methods combined with N-terminal sequencing. AAI is the shortest α-amylase inhibitor described so far which has no known close homologs in the sequence data bases. Its residue conservation patterns and disulfide connectivity are related to the squash family of proteinase inhibitors, to the cellulose binding domain of cellobiohydrolase, and to ω-conotoxin, i.e. a group of small proteins termed 'knottins' by Nguyen, D. L., Heitz, A., Chiche, L., Castro, B., Boigegrain, R., Favel, A., and Coletti-Previero, M. ((1990) (Biochimie 72, 431-435) The three-dimensional model of AAI was built according to the common structural features of this group of proteins using side-chain replacement and molecular dynamics refinement techniques.

Original languageEnglish
Pages (from-to)23675-23680
Number of pages6
JournalJournal of Biological Chemistry
Volume269
Issue number38
Publication statusPublished - Sep 23 1994

Fingerprint

Amaranthus
Amylases
Seed
Seeds
Disulfides
Cystine-Knot Miniproteins
Peptide Hydrolases
Cellulose 1,4-beta-Cellobiosidase
Conotoxins
Tribolium
Cucurbita
Molecular Dynamics Simulation
Sequence Homology
Cellulose
Crops
Larva
Insects
Molecular dynamics
Conservation
Proteins

ASJC Scopus subject areas

  • Biochemistry

Cite this

Chagolla-Lopez, A., Blanco-Labra, A., Pátthy, A., Sánchez, R., & Pongor, S. (1994). A novel α-amylase inhibitor from amaranth (Amaranthus hypocondriacus) seeds. Journal of Biological Chemistry, 269(38), 23675-23680.

A novel α-amylase inhibitor from amaranth (Amaranthus hypocondriacus) seeds. / Chagolla-Lopez, Alicia; Blanco-Labra, Alejandro; Pátthy, A.; Sánchez, Roberto; Pongor, Sándor.

In: Journal of Biological Chemistry, Vol. 269, No. 38, 23.09.1994, p. 23675-23680.

Research output: Contribution to journalArticle

Chagolla-Lopez, A, Blanco-Labra, A, Pátthy, A, Sánchez, R & Pongor, S 1994, 'A novel α-amylase inhibitor from amaranth (Amaranthus hypocondriacus) seeds', Journal of Biological Chemistry, vol. 269, no. 38, pp. 23675-23680.
Chagolla-Lopez A, Blanco-Labra A, Pátthy A, Sánchez R, Pongor S. A novel α-amylase inhibitor from amaranth (Amaranthus hypocondriacus) seeds. Journal of Biological Chemistry. 1994 Sep 23;269(38):23675-23680.
Chagolla-Lopez, Alicia ; Blanco-Labra, Alejandro ; Pátthy, A. ; Sánchez, Roberto ; Pongor, Sándor. / A novel α-amylase inhibitor from amaranth (Amaranthus hypocondriacus) seeds. In: Journal of Biological Chemistry. 1994 ; Vol. 269, No. 38. pp. 23675-23680.
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