A non-invasive assay of the plastoquinone pool redox state based on the OJIP-transient

Szilvia Z. Tóth, G. Schansker, Reto J. Strasser

Research output: Contribution to journalArticle

100 Citations (Scopus)

Abstract

The plastoquinone (PQ) pool of the photosynthetic electron transport chain becomes reduced under anaerobic conditions. Here, anaerobiosis was used as a tool to manipulate the PQ-pool redox state in darkness and to study the effects of the PQ-redox state on the Chl-a fluorescence (OJIP) kinetics in pea leaves (Pisum sativum L.). It is shown that the FJ (fluorescence intensity at 3 ms) is linearly related to the area above the OJ-phase (first 3 ms) representing the reduction of the acceptor side of photosystem II (PSII) and FJ is also linearly related to the area above the JI-phase (3-30 ms) that parallels the reduction of the PQ-pool. This means that FJ depends on the availability of oxidized PQ-molecules bound to the Q B-site. The linear relationships between FJ and the two areas indicate that FJ is not sensitive to energy transfer between PSII-antennae (connectivity). It is further shown that a ∼94% reduced PQ-pool is in equilibrium with a ∼19% reduction of QA (primary quinone acceptor of PSII). The non-linear relationship between the initial fluorescence value (F20 μs) and the area above the OJ-phase supports the idea that F20 μs is sensitive to connectivity. This is reinforced by the observation that this non-linearity can be overcome by transforming the F20 μs-values into [QA -]-values. Based on the FJ-value of the OJIP-transient, a simple method for the quantification of the redox state of the PQ-pool is proposed.

Original languageEnglish
Pages (from-to)193-203
Number of pages11
JournalPhotosynthesis Research
Volume93
Issue number1-3
DOIs
Publication statusPublished - Jul 2007

Fingerprint

Plastoquinone
photosystem II
Oxidation-Reduction
Assays
fluorescence
assays
Photosystem II Protein Complex
anaerobiosis
electron transport chain
energy transfer
quinones
Fluorescence
anaerobic conditions
Peas
antennae
Pisum sativum
peas
kinetics
Anaerobiosis
Darkness

Keywords

  • 820 nm transmission
  • Anaerobiosis
  • OJIP-transient
  • Pisum sativum L.
  • Plastoquinone pool

ASJC Scopus subject areas

  • Plant Science

Cite this

A non-invasive assay of the plastoquinone pool redox state based on the OJIP-transient. / Tóth, Szilvia Z.; Schansker, G.; Strasser, Reto J.

In: Photosynthesis Research, Vol. 93, No. 1-3, 07.2007, p. 193-203.

Research output: Contribution to journalArticle

Tóth, Szilvia Z. ; Schansker, G. ; Strasser, Reto J. / A non-invasive assay of the plastoquinone pool redox state based on the OJIP-transient. In: Photosynthesis Research. 2007 ; Vol. 93, No. 1-3. pp. 193-203.
@article{b8692cdab5544ec0bbf6dbc58523104b,
title = "A non-invasive assay of the plastoquinone pool redox state based on the OJIP-transient",
abstract = "The plastoquinone (PQ) pool of the photosynthetic electron transport chain becomes reduced under anaerobic conditions. Here, anaerobiosis was used as a tool to manipulate the PQ-pool redox state in darkness and to study the effects of the PQ-redox state on the Chl-a fluorescence (OJIP) kinetics in pea leaves (Pisum sativum L.). It is shown that the FJ (fluorescence intensity at 3 ms) is linearly related to the area above the OJ-phase (first 3 ms) representing the reduction of the acceptor side of photosystem II (PSII) and FJ is also linearly related to the area above the JI-phase (3-30 ms) that parallels the reduction of the PQ-pool. This means that FJ depends on the availability of oxidized PQ-molecules bound to the Q B-site. The linear relationships between FJ and the two areas indicate that FJ is not sensitive to energy transfer between PSII-antennae (connectivity). It is further shown that a ∼94{\%} reduced PQ-pool is in equilibrium with a ∼19{\%} reduction of QA (primary quinone acceptor of PSII). The non-linear relationship between the initial fluorescence value (F20 μs) and the area above the OJ-phase supports the idea that F20 μs is sensitive to connectivity. This is reinforced by the observation that this non-linearity can be overcome by transforming the F20 μs-values into [QA -]-values. Based on the FJ-value of the OJIP-transient, a simple method for the quantification of the redox state of the PQ-pool is proposed.",
keywords = "820 nm transmission, Anaerobiosis, OJIP-transient, Pisum sativum L., Plastoquinone pool",
author = "T{\'o}th, {Szilvia Z.} and G. Schansker and Strasser, {Reto J.}",
year = "2007",
month = "7",
doi = "10.1007/s11120-007-9179-8",
language = "English",
volume = "93",
pages = "193--203",
journal = "Photosynthesis Research",
issn = "0166-8595",
publisher = "Springer Netherlands",
number = "1-3",

}

TY - JOUR

T1 - A non-invasive assay of the plastoquinone pool redox state based on the OJIP-transient

AU - Tóth, Szilvia Z.

AU - Schansker, G.

AU - Strasser, Reto J.

PY - 2007/7

Y1 - 2007/7

N2 - The plastoquinone (PQ) pool of the photosynthetic electron transport chain becomes reduced under anaerobic conditions. Here, anaerobiosis was used as a tool to manipulate the PQ-pool redox state in darkness and to study the effects of the PQ-redox state on the Chl-a fluorescence (OJIP) kinetics in pea leaves (Pisum sativum L.). It is shown that the FJ (fluorescence intensity at 3 ms) is linearly related to the area above the OJ-phase (first 3 ms) representing the reduction of the acceptor side of photosystem II (PSII) and FJ is also linearly related to the area above the JI-phase (3-30 ms) that parallels the reduction of the PQ-pool. This means that FJ depends on the availability of oxidized PQ-molecules bound to the Q B-site. The linear relationships between FJ and the two areas indicate that FJ is not sensitive to energy transfer between PSII-antennae (connectivity). It is further shown that a ∼94% reduced PQ-pool is in equilibrium with a ∼19% reduction of QA (primary quinone acceptor of PSII). The non-linear relationship between the initial fluorescence value (F20 μs) and the area above the OJ-phase supports the idea that F20 μs is sensitive to connectivity. This is reinforced by the observation that this non-linearity can be overcome by transforming the F20 μs-values into [QA -]-values. Based on the FJ-value of the OJIP-transient, a simple method for the quantification of the redox state of the PQ-pool is proposed.

AB - The plastoquinone (PQ) pool of the photosynthetic electron transport chain becomes reduced under anaerobic conditions. Here, anaerobiosis was used as a tool to manipulate the PQ-pool redox state in darkness and to study the effects of the PQ-redox state on the Chl-a fluorescence (OJIP) kinetics in pea leaves (Pisum sativum L.). It is shown that the FJ (fluorescence intensity at 3 ms) is linearly related to the area above the OJ-phase (first 3 ms) representing the reduction of the acceptor side of photosystem II (PSII) and FJ is also linearly related to the area above the JI-phase (3-30 ms) that parallels the reduction of the PQ-pool. This means that FJ depends on the availability of oxidized PQ-molecules bound to the Q B-site. The linear relationships between FJ and the two areas indicate that FJ is not sensitive to energy transfer between PSII-antennae (connectivity). It is further shown that a ∼94% reduced PQ-pool is in equilibrium with a ∼19% reduction of QA (primary quinone acceptor of PSII). The non-linear relationship between the initial fluorescence value (F20 μs) and the area above the OJ-phase supports the idea that F20 μs is sensitive to connectivity. This is reinforced by the observation that this non-linearity can be overcome by transforming the F20 μs-values into [QA -]-values. Based on the FJ-value of the OJIP-transient, a simple method for the quantification of the redox state of the PQ-pool is proposed.

KW - 820 nm transmission

KW - Anaerobiosis

KW - OJIP-transient

KW - Pisum sativum L.

KW - Plastoquinone pool

UR - http://www.scopus.com/inward/record.url?scp=34548175269&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=34548175269&partnerID=8YFLogxK

U2 - 10.1007/s11120-007-9179-8

DO - 10.1007/s11120-007-9179-8

M3 - Article

VL - 93

SP - 193

EP - 203

JO - Photosynthesis Research

JF - Photosynthesis Research

SN - 0166-8595

IS - 1-3

ER -