A New Zearalenone Biodegradation Strategy Using Non-Pathogenic Rhodococcus pyridinivorans K408 Strain

Rókus Kriszt, Csilla Krifaton, Sándor Szoboszlay, Mátyás Cserháti, Balázs Kriszt, József Kukolya, Árpád Czéh, Szilvia Fehér-Tóth, Lívia Török, Zsuzsanna Szoke, K. Kovács, Teréz Barna, Szilamér Ferenczi

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Zearalenone (hereafter referred to as ZEA) is a nonsteroidal estrogenic mycotoxin produced by several Fusarium spp. on cereal grains. ZEA is one of the most hazardous natural endocrine disrupting chemicals (EDC) which induces hyper estrogenic responses in mammals. This can result in reproductive disorders in farm animals as well as in humans. Consequently, detoxification strategies for contaminated crops are crucial for food safety. In this study we have developed a bacterial based detoxification system using a non-pathogen Rhodococcus pyridinivorans K408 strain. Following 5 days treatment of ZEA with R. pyridinivorans K408 strain HPLC analyses showed an 87.21% ZEA-degradation efficiency of the bacterial enzyme systems. In another approach, the strain biotransformation ability has also been confirmed by a bioluminescent version of the yeast estrogen screening system (BLYES), which detected an 81.75% of biodegradability of ZEA, in a good agreement with the chemical analyses. Furthermore, the capacity of R. pyridinivorans to eliminate the estrogenic effects of ZEA was tested by using an immature uterotrophic assay. Prepubertal female rats were treated with vehicle (olive oil), 17β-estradiol, ZEA (0.1-1-5-10 mg/kg body weight) and LB broth containing 500 mg/l ZEA that has already been incubated with or without Rhodococcus pyridinivorans K408 strain. Uterine weights were measured and the mRNA level changes relating to apelin, aquaporin 5, complement component 2, and calbindin-3 genes were measured by qRT-PCR. These genes represent the major pathways that are affected by estromimetic compounds. Zearalenone feeding significantly increased the uterus weight in a dose dependent manner and at the same time upregulated complement component 2 and calbindin-3 expression as well as decreased apelin and aquaporin 5 mRNA levels comparable to that seen in 17β-estradiol exposed rats. In contrast, LB broth in which ZEA was incubated with Rhodococcus pyridinivorans K408 prior to the feeding did not display any estrogenic effect neither on uterine weight nor on the expression of estrogen-regulated genes. Consequently, the identification of Rhodococcus pyridinivorans K408 strain in ZEA biodegradation proved to be a very efficient biological tool that is able to eliminate the complete estrogenic effects of ZEA. It is also remarkable that this biotransformation pathway of ZEA did not result in any residual estrogenic effects.

Original languageEnglish
Article numbere43608
JournalPLoS One
Volume7
Issue number9
DOIs
Publication statusPublished - Sep 25 2012

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Zearalenone
Rhodococcus
zearalenone
Biodegradation
estrogenic properties
biodegradation
Estrogens
calbindin
Aquaporin 5
S100 Calcium Binding Protein G
Complement C2
aquaporins
Detoxification
biotransformation
Genes
estrogens
estradiol
Biotransformation
complement
Weights and Measures

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Kriszt, R., Krifaton, C., Szoboszlay, S., Cserháti, M., Kriszt, B., Kukolya, J., ... Ferenczi, S. (2012). A New Zearalenone Biodegradation Strategy Using Non-Pathogenic Rhodococcus pyridinivorans K408 Strain. PLoS One, 7(9), [e43608]. https://doi.org/10.1371/journal.pone.0043608

A New Zearalenone Biodegradation Strategy Using Non-Pathogenic Rhodococcus pyridinivorans K408 Strain. / Kriszt, Rókus; Krifaton, Csilla; Szoboszlay, Sándor; Cserháti, Mátyás; Kriszt, Balázs; Kukolya, József; Czéh, Árpád; Fehér-Tóth, Szilvia; Török, Lívia; Szoke, Zsuzsanna; Kovács, K.; Barna, Teréz; Ferenczi, Szilamér.

In: PLoS One, Vol. 7, No. 9, e43608, 25.09.2012.

Research output: Contribution to journalArticle

Kriszt, R, Krifaton, C, Szoboszlay, S, Cserháti, M, Kriszt, B, Kukolya, J, Czéh, Á, Fehér-Tóth, S, Török, L, Szoke, Z, Kovács, K, Barna, T & Ferenczi, S 2012, 'A New Zearalenone Biodegradation Strategy Using Non-Pathogenic Rhodococcus pyridinivorans K408 Strain', PLoS One, vol. 7, no. 9, e43608. https://doi.org/10.1371/journal.pone.0043608
Kriszt R, Krifaton C, Szoboszlay S, Cserháti M, Kriszt B, Kukolya J et al. A New Zearalenone Biodegradation Strategy Using Non-Pathogenic Rhodococcus pyridinivorans K408 Strain. PLoS One. 2012 Sep 25;7(9). e43608. https://doi.org/10.1371/journal.pone.0043608
Kriszt, Rókus ; Krifaton, Csilla ; Szoboszlay, Sándor ; Cserháti, Mátyás ; Kriszt, Balázs ; Kukolya, József ; Czéh, Árpád ; Fehér-Tóth, Szilvia ; Török, Lívia ; Szoke, Zsuzsanna ; Kovács, K. ; Barna, Teréz ; Ferenczi, Szilamér. / A New Zearalenone Biodegradation Strategy Using Non-Pathogenic Rhodococcus pyridinivorans K408 Strain. In: PLoS One. 2012 ; Vol. 7, No. 9.
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