A new validated high-performance liquid chromatographic method for the determination of EGIS-9933 in rat plasma

K. Balogh Nemes, J. Szúnyog, I. Klebovich

Research output: Contribution to journalArticle

Abstract

A new highly sensitive high-performance liquid chromatographic (HPLC) procedure for determination of EGIS-9933 (a newly developed anxiolytic compound) in rat plasma is described. A gradient, elution method with UV detection at 270 nm has been developed using a mobile phase of a mixture of A: methanol:acetonitrile 1:9 and B: 0.5 % triethilamine in water, the pH of B was adjusted to 3 with phosphoric acid. Solid phase extraction (SPE) was used for the sample preparation. The calibration was linear in the 10-10000 ng mL-1 concentration range. The limit of quantification was 10 ng mL-1. The bioanalytical method was validated according to internationally accepted criteria for biological samples.

Original languageEnglish
Pages (from-to)158-162
Number of pages5
JournalChromatographia
Volume48
Issue number1-2
DOIs
Publication statusPublished - 1998

Fingerprint

Gradient methods
Anti-Anxiety Agents
Methanol
Rats
Calibration
Plasmas
Water
Liquids
Solid Phase Extraction
phosphoric acid
acetonitrile

Keywords

  • Column liquid chromatography
  • EGIS-9933
  • Rat plasma
  • Solid phase extraction

ASJC Scopus subject areas

  • Analytical Chemistry
  • Clinical Biochemistry

Cite this

A new validated high-performance liquid chromatographic method for the determination of EGIS-9933 in rat plasma. / Balogh Nemes, K.; Szúnyog, J.; Klebovich, I.

In: Chromatographia, Vol. 48, No. 1-2, 1998, p. 158-162.

Research output: Contribution to journalArticle

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