A new type of enzyme electrode: The ascorbic acid eliminator electrode

Geza Nagy, Margaret E. Rice, Ralph N. Adams

Research output: Contribution to journalArticle

70 Citations (Scopus)

Abstract

A new type of enzyme electrode has been developed for in vivo electrochemical measurements which allows discrimination between ascorbic acid and catecholamines and their metabolites. The electrode employs the enzyme ascorbic acid oxidase held between the voltammetric electrode and solution by a dialysis membrane or immobilized on the outer surface of serous membrane from rat small intestine. The electrode gives linear calibration curves for all catecholamines and metabolites independent of any ascorbic acid concentrations significant in physiological measurements. The electrode has been tested in brain slice measurements and shown to respond to releases of catecholamines initiated by potassium ion stimulation.

Original languageEnglish
Pages (from-to)2611-2616
Number of pages6
JournalLife sciences
Volume31
Issue number23
DOIs
Publication statusPublished - Dec 6 1982

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Pharmacology, Toxicology and Pharmaceutics(all)

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