A new technique for the direct demonstration of overlapping cutaneous innervation territories of peptidergic C-fibre afferents of rat hindlimb nerves

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

A new technique based on the phenomenon of vascular labelling has been devised for the direct visualisation of overlapping innervation territories of cutaneous nerves. The saphenous, peroneal and sural nerves on one side in anaesthetised rats were exposed, cut centrally and successively stimulated antidromically to induce a neurogenic inflammatory response after an intravenous injection of either a 1% colloidal silver solution or a suspension of 3% Monastral Blue B. Light microscopic examination of transparent preparations of the dorsal hindpaw skin revealed labelled blood vessels of different colours which represented cutaneous territories served by different nerves. Blood vessels labelled with both substances were regarded as areas of overlapping innervation. Such areas were typically localised along the border of adjacent innervation territories. In addition, distinct areas exhibiting double-labelled blood vessels were regularly encountered in regions separate from this border zone. Areas of interest were drawn with the aid of a camera lucida and measured by means of a computerised system. The results indicate a significant, although topographically variable, degree of overlap of these cutaneous innervation areas. This new technique offers a possibility to explore the importance of normally existing overlap in the reinnervation of a denervated skin area by collateral nerve sprouting.

Original languageEnglish
Pages (from-to)47-52
Number of pages6
JournalJournal of Neuroscience Methods
Volume55
Issue number1
DOIs
Publication statusPublished - Nov 1994

Keywords

  • Capsaicin-sensitive
  • Colloidal silver
  • Cutaneous innervation area
  • Monastral Blue B
  • Neurogenic inflammation
  • Overlapping cutaneous innervation
  • Sensory nerve
  • Vascular labelling

ASJC Scopus subject areas

  • Neuroscience(all)

Fingerprint Dive into the research topics of 'A new technique for the direct demonstration of overlapping cutaneous innervation territories of peptidergic C-fibre afferents of rat hindlimb nerves'. Together they form a unique fingerprint.

  • Cite this