A new technique for the direct demonstration of overlapping cutaneous innervation territories of peptidergic C-fibre afferents of rat hindlimb nerves

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

A new technique based on the phenomenon of vascular labelling has been devised for the direct visualisation of overlapping innervation territories of cutaneous nerves. The saphenous, peroneal and sural nerves on one side in anaesthetised rats were exposed, cut centrally and successively stimulated antidromically to induce a neurogenic inflammatory response after an intravenous injection of either a 1% colloidal silver solution or a suspension of 3% Monastral Blue B. Light microscopic examination of transparent preparations of the dorsal hindpaw skin revealed labelled blood vessels of different colours which represented cutaneous territories served by different nerves. Blood vessels labelled with both substances were regarded as areas of overlapping innervation. Such areas were typically localised along the border of adjacent innervation territories. In addition, distinct areas exhibiting double-labelled blood vessels were regularly encountered in regions separate from this border zone. Areas of interest were drawn with the aid of a camera lucida and measured by means of a computerised system. The results indicate a significant, although topographically variable, degree of overlap of these cutaneous innervation areas. This new technique offers a possibility to explore the importance of normally existing overlap in the reinnervation of a denervated skin area by collateral nerve sprouting.

Original languageEnglish
Pages (from-to)47-52
Number of pages6
JournalJournal of Neuroscience Methods
Volume55
Issue number1
DOIs
Publication statusPublished - 1994

Fingerprint

Unmyelinated Nerve Fibers
Hindlimb
Blood Vessels
Skin
Sural Nerve
Peroneal Nerve
Intravenous Injections
Suspensions
Color
Light

Keywords

  • Capsaicin-sensitive
  • Colloidal silver
  • Cutaneous innervation area
  • Monastral Blue B
  • Neurogenic inflammation
  • Overlapping cutaneous innervation
  • Sensory nerve
  • Vascular labelling

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

@article{2fbabe4ea8684fafb63322840de0139e,
title = "A new technique for the direct demonstration of overlapping cutaneous innervation territories of peptidergic C-fibre afferents of rat hindlimb nerves",
abstract = "A new technique based on the phenomenon of vascular labelling has been devised for the direct visualisation of overlapping innervation territories of cutaneous nerves. The saphenous, peroneal and sural nerves on one side in anaesthetised rats were exposed, cut centrally and successively stimulated antidromically to induce a neurogenic inflammatory response after an intravenous injection of either a 1{\%} colloidal silver solution or a suspension of 3{\%} Monastral Blue B. Light microscopic examination of transparent preparations of the dorsal hindpaw skin revealed labelled blood vessels of different colours which represented cutaneous territories served by different nerves. Blood vessels labelled with both substances were regarded as areas of overlapping innervation. Such areas were typically localised along the border of adjacent innervation territories. In addition, distinct areas exhibiting double-labelled blood vessels were regularly encountered in regions separate from this border zone. Areas of interest were drawn with the aid of a camera lucida and measured by means of a computerised system. The results indicate a significant, although topographically variable, degree of overlap of these cutaneous innervation areas. This new technique offers a possibility to explore the importance of normally existing overlap in the reinnervation of a denervated skin area by collateral nerve sprouting.",
keywords = "Capsaicin-sensitive, Colloidal silver, Cutaneous innervation area, Monastral Blue B, Neurogenic inflammation, Overlapping cutaneous innervation, Sensory nerve, Vascular labelling",
author = "M. Dux and G. Jancs{\'o}",
year = "1994",
doi = "10.1016/0165-0270(94)90039-6",
language = "English",
volume = "55",
pages = "47--52",
journal = "Journal of Neuroscience Methods",
issn = "0165-0270",
publisher = "Elsevier",
number = "1",

}

TY - JOUR

T1 - A new technique for the direct demonstration of overlapping cutaneous innervation territories of peptidergic C-fibre afferents of rat hindlimb nerves

AU - Dux, M.

AU - Jancsó, G.

PY - 1994

Y1 - 1994

N2 - A new technique based on the phenomenon of vascular labelling has been devised for the direct visualisation of overlapping innervation territories of cutaneous nerves. The saphenous, peroneal and sural nerves on one side in anaesthetised rats were exposed, cut centrally and successively stimulated antidromically to induce a neurogenic inflammatory response after an intravenous injection of either a 1% colloidal silver solution or a suspension of 3% Monastral Blue B. Light microscopic examination of transparent preparations of the dorsal hindpaw skin revealed labelled blood vessels of different colours which represented cutaneous territories served by different nerves. Blood vessels labelled with both substances were regarded as areas of overlapping innervation. Such areas were typically localised along the border of adjacent innervation territories. In addition, distinct areas exhibiting double-labelled blood vessels were regularly encountered in regions separate from this border zone. Areas of interest were drawn with the aid of a camera lucida and measured by means of a computerised system. The results indicate a significant, although topographically variable, degree of overlap of these cutaneous innervation areas. This new technique offers a possibility to explore the importance of normally existing overlap in the reinnervation of a denervated skin area by collateral nerve sprouting.

AB - A new technique based on the phenomenon of vascular labelling has been devised for the direct visualisation of overlapping innervation territories of cutaneous nerves. The saphenous, peroneal and sural nerves on one side in anaesthetised rats were exposed, cut centrally and successively stimulated antidromically to induce a neurogenic inflammatory response after an intravenous injection of either a 1% colloidal silver solution or a suspension of 3% Monastral Blue B. Light microscopic examination of transparent preparations of the dorsal hindpaw skin revealed labelled blood vessels of different colours which represented cutaneous territories served by different nerves. Blood vessels labelled with both substances were regarded as areas of overlapping innervation. Such areas were typically localised along the border of adjacent innervation territories. In addition, distinct areas exhibiting double-labelled blood vessels were regularly encountered in regions separate from this border zone. Areas of interest were drawn with the aid of a camera lucida and measured by means of a computerised system. The results indicate a significant, although topographically variable, degree of overlap of these cutaneous innervation areas. This new technique offers a possibility to explore the importance of normally existing overlap in the reinnervation of a denervated skin area by collateral nerve sprouting.

KW - Capsaicin-sensitive

KW - Colloidal silver

KW - Cutaneous innervation area

KW - Monastral Blue B

KW - Neurogenic inflammation

KW - Overlapping cutaneous innervation

KW - Sensory nerve

KW - Vascular labelling

UR - http://www.scopus.com/inward/record.url?scp=0028117604&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028117604&partnerID=8YFLogxK

U2 - 10.1016/0165-0270(94)90039-6

DO - 10.1016/0165-0270(94)90039-6

M3 - Article

C2 - 7891461

AN - SCOPUS:0028117604

VL - 55

SP - 47

EP - 52

JO - Journal of Neuroscience Methods

JF - Journal of Neuroscience Methods

SN - 0165-0270

IS - 1

ER -