A new simplified assay for evaluation of motile activity of human polymorphonuclear leucocytes

L. Bogár, Z. Molnár, M. Tekeres

Research output: Contribution to journalArticle

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Abstract

We studied the adaptation of a filtration instrument to an assay for cell migration experiments. The commercially available Nuclepore filter holders were modified so that isolated human polymorphonuclear leucocytes (PMNs) were allowed to penetrate a porous filter membrane and to enter a compartment under the membrane. The total number of PMNs which had passed the pores during an hour of incubation could be counted. In this way we carried out experiments using Shigella sonnei lipopolysaccharide and dilutions of E. coli culture supernatant as chemoattractants for healthy human PMNs. These studies showed that the method can distinguish between random and directed movements of PMNs and it is sensitive to the concentrations of chemoattractant. Furthermore, the data obtained using PMNs from the same subject on different days seem to be comparable.

Original languageEnglish
Pages (from-to)211-215
Number of pages5
JournalImmunology Letters
Volume17
Issue number3
DOIs
Publication statusPublished - 1988

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Chemotactic Factors
Human Activities
Neutrophils
Cell Migration Assays
Shigella sonnei
Membranes
Lipopolysaccharides
Escherichia coli

Keywords

  • Endotoxin
  • Neutrophil chemotaxis
  • Random and directed movements

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy

Cite this

A new simplified assay for evaluation of motile activity of human polymorphonuclear leucocytes. / Bogár, L.; Molnár, Z.; Tekeres, M.

In: Immunology Letters, Vol. 17, No. 3, 1988, p. 211-215.

Research output: Contribution to journalArticle

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