A new sequence-specific endonuclease (Bsp) from Bacillus sphaericus

A. Kiss, Béla Sain, Éva Csordás-Tòth, Pál Venetianer

Research output: Contribution to journalArticle

47 Citations (Scopus)

Abstract

A new restriction endonuclease has been isolated from Bacillus sphaericus R. The purification procedure includes Bio-Gel filtration, (NH4)2SO4 fractionation and phosphocellulose chromatography. After the phosphocellulose step the enzyme preparation is free of non-specific nucleases. Bsp cleaves double-stranded DNA with the same specificity as Bacillus subtilis (Bsu) and Haemophilus aegyptius (HaeIII) restriction endonucleases, as concluded from digests and double-digests of φX174 replicative form DNA with Bsu and Bsp. The 5′-terminal nucleotide of the cleavage products was shown to be C. Bacillus sphaericus R produces Bsp in extremely large quantities and the enzyme can be easily purified in high yield.

Original languageEnglish
Pages (from-to)323-329
Number of pages7
JournalGene
Volume1
Issue number5-6
DOIs
Publication statusPublished - 1977

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DNA Restriction Enzymes
Bacillus subtilis
Bacillus
Haemophilus
DNA
Enzymes
Gel Chromatography
Chromatography
Nucleotides
phosphocellulose
endodeoxyribonuclease Bsp
GGCC-specific type II deoxyribonucleases

ASJC Scopus subject areas

  • Genetics

Cite this

A new sequence-specific endonuclease (Bsp) from Bacillus sphaericus. / Kiss, A.; Sain, Béla; Csordás-Tòth, Éva; Venetianer, Pál.

In: Gene, Vol. 1, No. 5-6, 1977, p. 323-329.

Research output: Contribution to journalArticle

Kiss, A, Sain, B, Csordás-Tòth, É & Venetianer, P 1977, 'A new sequence-specific endonuclease (Bsp) from Bacillus sphaericus', Gene, vol. 1, no. 5-6, pp. 323-329. https://doi.org/10.1016/0378-1119(77)90037-3
Kiss, A. ; Sain, Béla ; Csordás-Tòth, Éva ; Venetianer, Pál. / A new sequence-specific endonuclease (Bsp) from Bacillus sphaericus. In: Gene. 1977 ; Vol. 1, No. 5-6. pp. 323-329.
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