A new, modern, cost-saving micro/macro method for the determination of serum fructosamine

Klára Oppel, M. Kulcsár, L. Bárdos, A. Ferencz, Hajnalka Lakner, Judit Simon, Kriszta Temesváry, Krisztina Karchesz

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Serum/plasma fructosamine (SeFa) concentration is a reliable indicator used in human diabetic control. Tests for monitoring the carbohydrate/energy metabolism of (farm) animals are less commonly performed in veterinary laboratories, since most of the reliable determinations, both automated and manual, are relatively expensive. The aim of this study was to develop a precise, money- (and time-) saving automated micro method for measuring SeFa. ELISA microplates (20 μL samples and 200 μL reagents) and an automatic microplate autoreader were used. The classical nitroblue tetrazolium (NBT) stain reagent solution of Johnson et al. (1982) was modified using a SIGMA reagent to render it stable for up to one year, SeFa concentrations measured by the new method in 30 human blood plasma samples were compared with values obtained by the standard (generally used) LaRoche kit procedure. Fifteen cow, 13 dog and 18 chicken plasma samples were assayed by the new automated 'micro' method as well as by the manual test tube 'macro' method commonly used earlier. The modified reagent was applied for both methods. The coefficient of correlation (r) between the results obtained by the two methods was consistently between 0.94 and 0.98 (p

Original languageEnglish
Pages (from-to)285-291
Number of pages7
JournalActa Veterinaria Hungarica
Volume48
Issue number3
Publication statusPublished - 2000

Fingerprint

Fructosamine
cost effectiveness
blood serum
Costs and Cost Analysis
Serum
methodology
nitroblue tetrazolium
Nitroblue Tetrazolium
carbohydrate metabolism
farmed animal species
blood plasma
sampling
Domestic Animals
Carbohydrate Metabolism
energy metabolism
dyes
Energy Metabolism
testing
Chickens
enzyme-linked immunosorbent assay

Keywords

  • Cost-saving modified reagent
  • Glycated haemoglobin
  • Glycated proteins
  • New automated micro method
  • Serum fructosamine

ASJC Scopus subject areas

  • veterinary(all)

Cite this

Oppel, K., Kulcsár, M., Bárdos, L., Ferencz, A., Lakner, H., Simon, J., ... Karchesz, K. (2000). A new, modern, cost-saving micro/macro method for the determination of serum fructosamine. Acta Veterinaria Hungarica, 48(3), 285-291.

A new, modern, cost-saving micro/macro method for the determination of serum fructosamine. / Oppel, Klára; Kulcsár, M.; Bárdos, L.; Ferencz, A.; Lakner, Hajnalka; Simon, Judit; Temesváry, Kriszta; Karchesz, Krisztina.

In: Acta Veterinaria Hungarica, Vol. 48, No. 3, 2000, p. 285-291.

Research output: Contribution to journalArticle

Oppel, K, Kulcsár, M, Bárdos, L, Ferencz, A, Lakner, H, Simon, J, Temesváry, K & Karchesz, K 2000, 'A new, modern, cost-saving micro/macro method for the determination of serum fructosamine', Acta Veterinaria Hungarica, vol. 48, no. 3, pp. 285-291.
Oppel, Klára ; Kulcsár, M. ; Bárdos, L. ; Ferencz, A. ; Lakner, Hajnalka ; Simon, Judit ; Temesváry, Kriszta ; Karchesz, Krisztina. / A new, modern, cost-saving micro/macro method for the determination of serum fructosamine. In: Acta Veterinaria Hungarica. 2000 ; Vol. 48, No. 3. pp. 285-291.
@article{222c5aa63b6249adb22b436e5ff65e25,
title = "A new, modern, cost-saving micro/macro method for the determination of serum fructosamine",
abstract = "Serum/plasma fructosamine (SeFa) concentration is a reliable indicator used in human diabetic control. Tests for monitoring the carbohydrate/energy metabolism of (farm) animals are less commonly performed in veterinary laboratories, since most of the reliable determinations, both automated and manual, are relatively expensive. The aim of this study was to develop a precise, money- (and time-) saving automated micro method for measuring SeFa. ELISA microplates (20 μL samples and 200 μL reagents) and an automatic microplate autoreader were used. The classical nitroblue tetrazolium (NBT) stain reagent solution of Johnson et al. (1982) was modified using a SIGMA reagent to render it stable for up to one year, SeFa concentrations measured by the new method in 30 human blood plasma samples were compared with values obtained by the standard (generally used) LaRoche kit procedure. Fifteen cow, 13 dog and 18 chicken plasma samples were assayed by the new automated 'micro' method as well as by the manual test tube 'macro' method commonly used earlier. The modified reagent was applied for both methods. The coefficient of correlation (r) between the results obtained by the two methods was consistently between 0.94 and 0.98 (p",
keywords = "Cost-saving modified reagent, Glycated haemoglobin, Glycated proteins, New automated micro method, Serum fructosamine",
author = "Kl{\'a}ra Oppel and M. Kulcs{\'a}r and L. B{\'a}rdos and A. Ferencz and Hajnalka Lakner and Judit Simon and Kriszta Temesv{\'a}ry and Krisztina Karchesz",
year = "2000",
language = "English",
volume = "48",
pages = "285--291",
journal = "Acta Veterinaria Hungarica",
issn = "0236-6290",
publisher = "Akademiai Kiado",
number = "3",

}

TY - JOUR

T1 - A new, modern, cost-saving micro/macro method for the determination of serum fructosamine

AU - Oppel, Klára

AU - Kulcsár, M.

AU - Bárdos, L.

AU - Ferencz, A.

AU - Lakner, Hajnalka

AU - Simon, Judit

AU - Temesváry, Kriszta

AU - Karchesz, Krisztina

PY - 2000

Y1 - 2000

N2 - Serum/plasma fructosamine (SeFa) concentration is a reliable indicator used in human diabetic control. Tests for monitoring the carbohydrate/energy metabolism of (farm) animals are less commonly performed in veterinary laboratories, since most of the reliable determinations, both automated and manual, are relatively expensive. The aim of this study was to develop a precise, money- (and time-) saving automated micro method for measuring SeFa. ELISA microplates (20 μL samples and 200 μL reagents) and an automatic microplate autoreader were used. The classical nitroblue tetrazolium (NBT) stain reagent solution of Johnson et al. (1982) was modified using a SIGMA reagent to render it stable for up to one year, SeFa concentrations measured by the new method in 30 human blood plasma samples were compared with values obtained by the standard (generally used) LaRoche kit procedure. Fifteen cow, 13 dog and 18 chicken plasma samples were assayed by the new automated 'micro' method as well as by the manual test tube 'macro' method commonly used earlier. The modified reagent was applied for both methods. The coefficient of correlation (r) between the results obtained by the two methods was consistently between 0.94 and 0.98 (p

AB - Serum/plasma fructosamine (SeFa) concentration is a reliable indicator used in human diabetic control. Tests for monitoring the carbohydrate/energy metabolism of (farm) animals are less commonly performed in veterinary laboratories, since most of the reliable determinations, both automated and manual, are relatively expensive. The aim of this study was to develop a precise, money- (and time-) saving automated micro method for measuring SeFa. ELISA microplates (20 μL samples and 200 μL reagents) and an automatic microplate autoreader were used. The classical nitroblue tetrazolium (NBT) stain reagent solution of Johnson et al. (1982) was modified using a SIGMA reagent to render it stable for up to one year, SeFa concentrations measured by the new method in 30 human blood plasma samples were compared with values obtained by the standard (generally used) LaRoche kit procedure. Fifteen cow, 13 dog and 18 chicken plasma samples were assayed by the new automated 'micro' method as well as by the manual test tube 'macro' method commonly used earlier. The modified reagent was applied for both methods. The coefficient of correlation (r) between the results obtained by the two methods was consistently between 0.94 and 0.98 (p

KW - Cost-saving modified reagent

KW - Glycated haemoglobin

KW - Glycated proteins

KW - New automated micro method

KW - Serum fructosamine

UR - http://www.scopus.com/inward/record.url?scp=0034576689&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0034576689&partnerID=8YFLogxK

M3 - Article

C2 - 11402712

AN - SCOPUS:0034576689

VL - 48

SP - 285

EP - 291

JO - Acta Veterinaria Hungarica

JF - Acta Veterinaria Hungarica

SN - 0236-6290

IS - 3

ER -