A new, modern, cost-saving micro/macro method for the determination of serum fructosamine

Klára Oppel, Margit Kulcsár, L. Bárdos, A. Ferencz, Hajnalka Lakner, Judit Simon, Kriszta Temesváry, Krisztina Karchesz

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Serum/plasma fructosamine (SeFa) concentration is a reliable indicator used in human diabetic control. Tests for monitoring the carbohydrate/energy metabolism of (farm) animals are less commonly performed in veterinary laboratories, since most of the reliable determinations, both automated and manual, are relatively expensive. The aim of this study was to develop a precise, money- (and time-) saving automated micro method for measuring SeFa. ELISA microplates (20 μL samples and 200 μL reagents) and an automatic microplate autoreader were used. The classical nitroblue tetrazolium (NBT) stain reagent solution of Johnson et al. (1982) was modified using a SIGMA reagent to render it stable for up to one year, SeFa concentrations measured by the new method in 30 human blood plasma samples were compared with values obtained by the standard (generally used) LaRoche kit procedure. Fifteen cow, 13 dog and 18 chicken plasma samples were assayed by the new automated 'micro' method as well as by the manual test tube 'macro' method commonly used earlier. The modified reagent was applied for both methods. The coefficient of correlation (r) between the results obtained by the two methods was consistently between 0.94 and 0.98 (p <0.001).

Original languageEnglish
Pages (from-to)285-291
Number of pages7
JournalActa veterinaria Hungarica
Volume48
Issue number3
DOIs
Publication statusPublished - Jan 1 2000

Keywords

  • Cost-saving modified reagent
  • Glycated haemoglobin
  • Glycated proteins
  • New automated micro method
  • Serum fructosamine

ASJC Scopus subject areas

  • veterinary(all)

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  • Cite this

    Oppel, K., Kulcsár, M., Bárdos, L., Ferencz, A., Lakner, H., Simon, J., Temesváry, K., & Karchesz, K. (2000). A new, modern, cost-saving micro/macro method for the determination of serum fructosamine. Acta veterinaria Hungarica, 48(3), 285-291. https://doi.org/10.1556/AVet.48.2000.3.5