A new method to localize acid phosphatase using the confocal laser- scanning microscope

Zsolt Szegedi, Farhana Amin, Béla Szende, Ivor Delme Bowen

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

The aim of the study was to develop a technique for the detection of acid phosphatase enzyme activity by confocal laser-scanning microscope using the histochemical acid phosphatase detection method (after Barka and Anderson 1962, modified by Bowen and Lewis 1985) routinely used for light microscopy. The density and the distribution of enzyme reaction product is dependent on the incubation time, as shown by different confocal images or ELISA reader. The inhibition of the enzyme activity with metal ions shows the same profile known from the literature. This staining method seems to be useful to demonstrate subcellular distribution of the enzyme in the lysosomes and in the Golgi apparatus.

Original languageEnglish
Pages (from-to)217-223
Number of pages7
JournalPathology and Oncology Research
Volume4
Issue number3
DOIs
Publication statusPublished - Jan 1 1998

Keywords

  • Acid phosphatase activity
  • Cell image analysis
  • Confocal laser- scanning microscope
  • Cytometry

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Oncology
  • Cancer Research

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