A new method for amplification and sequence analysis of the full-length of HBV genome

Jin jun Guo, Ai long Huang, Z. Zhao, N. Tang, Hui min Shen

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

OBJECTIVES: To develop a new method for amplifying and sequencing the full-length of HBV genome. METHODS: A pair of primers located at the nick region of HBV molecule and a thermostable polymerase with high fidelity and sensitivity were used. After cloning the PCR products into a plasmid, the sequences of HBV genome were analyzed. RESULTS: The full-length of HBV genome were acquired using this method. The sensitivity and fidelity of the new method were also analyzed. The least quantity of initial templates was 10(2) and the artificial mutation rate was 1.2 bp/kb. CONCLUSION: This method can be used in amplification and sequence analysis of the full-length of HBV genome on a large scale.

Original languageEnglish
Pages (from-to)592-594
Number of pages3
JournalZhonghua gan zang bing za zhi = Zhonghua ganzangbing zazhi = Chinese journal of hepatology
Volume11
Issue number10
Publication statusPublished - 2003

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Sequence Analysis
Genome
Mutation Rate
Organism Cloning
Plasmids
Polymerase Chain Reaction

Cite this

A new method for amplification and sequence analysis of the full-length of HBV genome. / Guo, Jin jun; Huang, Ai long; Zhao, Z.; Tang, N.; Shen, Hui min.

In: Zhonghua gan zang bing za zhi = Zhonghua ganzangbing zazhi = Chinese journal of hepatology, Vol. 11, No. 10, 2003, p. 592-594.

Research output: Contribution to journalArticle

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AU - Zhao, Z.

AU - Tang, N.

AU - Shen, Hui min

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