A new insight into the zinc-dependent DNA-cleavage by the colicin E7 nuclease: A crystallographic and computational study

Anikó Czene, Eszter Tóth, Eszter Németh, Harm Otten, Jens Christian N Poulsen, Hans E M Christensen, Lubomír Rulíšek, Kyosuke Nagata, Sine Larsen, B. Gyurcsik

Research output: Contribution to journalArticle

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Abstract

The nuclease domain of colicin E7 metallonuclease (NColE7) contains its active centre at the C-terminus. The mutant ΔN4-NColE7-C∗-where the four N-terminal residues including the positively charged K446, R447 and K449 are replaced with eight residues from the GST tag-is catalytically inactive. The crystal structure of this mutant demonstrates that its overall fold is very similar to that of the native NColE7 structure. This implicates the stabilizing effect of the remaining N-terminal sequence on the structure of the C-terminal catalytic site and the essential role of the deleted residues in the mechanism of the catalyzed reaction. Complementary QM/MM calculations on the protein-DNA complexes support the less favourable cleavage by the mutant protein than by NColE7. Furthermore, a water molecule as a possible ligand for the Zn2+-ion is proposed to play a role in the catalytic process. These results suggest that the mechanism of the Zn2+-containing HNH nucleases needs to be further studied and discussed.

Original languageEnglish
Pages (from-to)2090-2099
Number of pages10
JournalMetallomics
Volume6
Issue number11
DOIs
Publication statusPublished - Nov 1 2014

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Colicins
DNA Cleavage
Mutant Proteins
Zinc
Catalytic Domain
DNA
Crystal structure
Ions
Ligands
Proteins
Molecules
Water

ASJC Scopus subject areas

  • Biomaterials
  • Metals and Alloys
  • Chemistry (miscellaneous)
  • Biochemistry
  • Biophysics
  • Medicine(all)

Cite this

A new insight into the zinc-dependent DNA-cleavage by the colicin E7 nuclease : A crystallographic and computational study. / Czene, Anikó; Tóth, Eszter; Németh, Eszter; Otten, Harm; Poulsen, Jens Christian N; Christensen, Hans E M; Rulíšek, Lubomír; Nagata, Kyosuke; Larsen, Sine; Gyurcsik, B.

In: Metallomics, Vol. 6, No. 11, 01.11.2014, p. 2090-2099.

Research output: Contribution to journalArticle

Czene, A, Tóth, E, Németh, E, Otten, H, Poulsen, JCN, Christensen, HEM, Rulíšek, L, Nagata, K, Larsen, S & Gyurcsik, B 2014, 'A new insight into the zinc-dependent DNA-cleavage by the colicin E7 nuclease: A crystallographic and computational study', Metallomics, vol. 6, no. 11, pp. 2090-2099. https://doi.org/10.1039/c4mt00195h
Czene, Anikó ; Tóth, Eszter ; Németh, Eszter ; Otten, Harm ; Poulsen, Jens Christian N ; Christensen, Hans E M ; Rulíšek, Lubomír ; Nagata, Kyosuke ; Larsen, Sine ; Gyurcsik, B. / A new insight into the zinc-dependent DNA-cleavage by the colicin E7 nuclease : A crystallographic and computational study. In: Metallomics. 2014 ; Vol. 6, No. 11. pp. 2090-2099.
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