A new homeotic mutation in the Drosophila bithorax complex remopves a boundary separating two domains of regulation

H. Gyurkovics, J. Gausz, J. Kummer, F. Karch

Research output: Contribution to journalArticle

165 Citations (Scopus)

Abstract

The bithorax complex specifies the identity of parasegments 5-14 of Drosphila. Although nine parasegment-specific functions, abx/bx, bxd/pbx and iab-2 to iab-8,9 have been identified, the whole bithorax complex appears to encode only three classes of proteins, Ubx, abd-A and Abd-B. Many observations suggest that the parasegment-specific functions act as positive cis-regulatory elements of Ubx, abd-A and Abd-B. We report the molecular genetics of a new gain-of-function mutation, Fab-7, which transforms parasegment 11 into parasegment 12. Induction of Abd-B mutations in cis (one of which removes the Abd-B homeobox) causes reversion of the dominant phenotype, demonstrating that Fab-7 misregulates Abd-B. A 4 kb deletion, 30 kb downstream from the Abd-B transcription unit, is solely responsible for the Fab-7 phenotype. We consider that the para-segment-specific functions lie in DNA domains that are sequentially and independently 'opened' along the chromosome. Once a domain is opened, the cis-regulatory sequences within it can carry out their function. We propose that the Fab-7 deletion removes a boundary separating the iab-6 and iab-7 cis-regulatory regions (the functions specific for parasegments 11 and 12) allowing the open configuration of iab-6 to invade iab-7 in parasegment 11. This is strongly supported by our finding that Fab-7 can be caused to revert by lesions not only in iab-7 but also in iab-6.

Original languageEnglish
Pages (from-to)2579-2585
Number of pages7
JournalEMBO Journal
Volume9
Issue number8
DOIs
Publication statusPublished - 1990

Keywords

  • AbdB
  • DNA boundary
  • bithorax complex
  • cis-regulation
  • gain-of-function mutation

ASJC Scopus subject areas

  • Neuroscience(all)
  • Molecular Biology
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

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