A new culture-free microbiotest for routine detection of cyanobacterial toxins

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

The Thamnotoxkit F(TM) was evaluated for detecting cyanobacterial toxins. This is a 24 hour bioassay using, as test organisms larvae of the freshwater anostracan crustacean Thamnocephalus platyurus hatched from cysts. Freeze-dried Microcystis aeruginosa samples from freshwaters throughout Hungary were tested. Good correlation was obtained between the results of intraperitoneal mouse bioassays and Thamnotoxkit F(TM) assays. The LC50's for purified microcystins- LR, -RR, and -YR ranged from 0.1 to 2.27 μg per mL, respectively in the Thamnotoxkit assay. The LC50 of laboratory cultures of Cylindrospermopsis raciborskii isolated from samples from Lake Balaton, was 0.574 mg lyophilized material per mL, in a four-laboratory trial. Although not developed originally for detecting cyanobacterial toxins, the Thamnotoxkit F(TM) has good potential as an initial screening procedure for toxicity assessment of cyanobacterial scums and blooms in freshwaters.

Original languageEnglish
Pages (from-to)466-472
Number of pages7
JournalEnvironmental Toxicology
Volume14
Issue number5
DOIs
Publication statusPublished - 1999

Fingerprint

Bioassay
Fresh Water
toxin
Assays
Biological Assay
bioassay
Cylindrospermopsis
assay
Microcystis
Toxicity
Lakes
Screening
Hungary
cyst
Larva
crustacean
Cysts
algal bloom
toxicity
larva

Keywords

  • Cyanobacterial toxins
  • Cylindrospermopsis raciborskii
  • Microcystis aeruginosa
  • Thamnotoxkit

ASJC Scopus subject areas

  • Environmental Science(all)
  • Environmental Chemistry
  • Health, Toxicology and Mutagenesis
  • Pollution
  • Water Science and Technology
  • Toxicology

Cite this

A new culture-free microbiotest for routine detection of cyanobacterial toxins. / Törökné, A.

In: Environmental Toxicology, Vol. 14, No. 5, 1999, p. 466-472.

Research output: Contribution to journalArticle

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