A new class of chemotactic peptides containing EWS motif: A mini-review

Eszter Illyés, Szilvia Bôsze, Orsolya Láng, Ferenc Sebestyén, László Kôhidai, Ferenc Hudecz

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

In this paper we describe the solid phase synthesis and chemical characterisation of oligopeptides corresponding to the IL-6 receptor (EWS, SEWS, WSEWS, EWSEWS and GEWSEWS) and two 19 member libraries (SXWS and WSXWS). Comparative analysis of the chemotactic properties of these compounds resulted in the identification of a highly active chemoattractant, peptide SEWS, which exhibits a prominent effect (660 ± 21%) at 1O-12 M on a ciliated protozoon, Tetrahymena pyriformis. We found that the chemotaxis of cells induced with the SXWS and WSXWS peptides varied according to the nature of the amino acid residue (Ala, Asp, Lys) in posìtion X. Ligand-binding experiments with N-terminally labelled fluorescent derivatives of SXWS peptides, applying [4-[7-hydroxycoumaryl]]acetic acid (Hca-OH) or 4-ethoxymethylene2-[1]-naphthyl-5(4H)-oxazolone (naOx-OEt) showed good correlation between binding profiles and chemotactic responsiveness (SEWS >SAWS > SDWS ~ SKWS). Chemotactic responsiveness and the binding characteristics data indicate that chemosensory activity is a surface-membrane associated event, and it is based on receptor-mediated signalling mechanisms.

Original languageEnglish
Pages (from-to)55-61
Number of pages7
JournalChimica Oggi
Volume20
Issue number7-8
Publication statusPublished - Jul 1 2002

ASJC Scopus subject areas

  • Chemistry(all)

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