A new, behaving, head restrained, eye movement-controlled feline model for chronic visual electrophysiological recordings

Tamás Nagypál, Péter Gombköto, Györgyi Utassy, Robert G. Averkin, György Benedek, Attila Nagy

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Background: Anesthetized, paralyzed domestic cats are often used as model organisms in visual neurophysiology. However, in the last few decades, behaving animal models have gathered ground in neurophysiology, due to their advantages over anesthetized, paralyzed models. New Method: In the present study a new, behaving, awake feline model is described, which is suitable for chronic visual electrophysiological recordings. Two trained, head- fixed cats were suspended in a canvas harness in a specially designed stand. The animals had been trained to fixate the center of a monitor during static and dynamic visual stimulation. Eye movements were monitored with implanted scleral coil in a magnetic field. Cell-level activity was recorded with eight electrodes implanted in the caudate nucleus. Results: Our two trained cats could maintain accurate fixation, even during optic flow stimulation, in an acceptance window of ±2.5° and ±1.5°, respectively. The model has yielded accurate recordings for over two years. Comparison with Existing Method(s): To our knowledge, this is the first awake, behaving feline model with rigorous eye movement control for chronic, cell-level visual electrophysiological recordings, which has actually proven to work during a longer period. Conclusions: The new model is optimal for chronic visual electrophysiological recordings in the awake, behaving domestic cat.

Original languageEnglish
Pages (from-to)1-7
Number of pages7
JournalJournal of Neuroscience Methods
Volume221
DOIs
Publication statusPublished - Jan 15 2014

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Keywords

  • Behavioral training
  • Fixation paradigm
  • Long-time recordings
  • Suspended cat
  • Visual electrophysiology

ASJC Scopus subject areas

  • Neuroscience(all)

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