A myosin II nanomachine mimicking the striated muscle

Irene Pertici, Lorenzo Bongini, Luca Melli, Giulio Bianchi, Luca Salvi, Giulia Falorsi, Caterina Squarci, Tamás Bozó, Dan Cojoc, M. Kellermayer, Vincenzo Lombardi, Pasquale Bianco

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The contraction of striated muscle (skeletal and cardiac muscle) is generated by ATP-dependent interactions between the molecular motor myosin II and the actin filament. The myosin motors are mechanically coupled along the thick filament in a geometry not achievable by single-molecule experiments. Here we show that a synthetic one-dimensional nanomachine, comprising fewer than ten myosin II dimers purified from rabbit psoas, performs isometric and isotonic contractions at 2 mM ATP, delivering a maximum power of 5 aW. The results are explained with a kinetic model fitted to the performance of mammalian skeletal muscle, showing that the condition for the motor coordination that maximises the efficiency in striated muscle is a minimum of 32 myosin heads sharing a common mechanical ground. The nanomachine offers a powerful tool for investigating muscle contractile-protein physiology, pathology and pharmacology without the potentially disturbing effects of the cytoskeletal—and regulatory—protein environment.

Original languageEnglish
Article number3532
JournalNature Communications
Volume9
Issue number1
DOIs
Publication statusPublished - Dec 1 2018

Fingerprint

Myosin Type II
myosins
skeletal muscle
Striated Muscle
Myosins
Muscle
Skeletal Muscle
Isotonic Contraction
Adenosine Triphosphate
Contractile Proteins
Isometric Contraction
Muscle Proteins
adenosine triphosphate
muscles
Actin Cytoskeleton
contraction
filaments
Myocardium
pharmacology
Pharmacology

ASJC Scopus subject areas

  • Chemistry(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Physics and Astronomy(all)

Cite this

Pertici, I., Bongini, L., Melli, L., Bianchi, G., Salvi, L., Falorsi, G., ... Bianco, P. (2018). A myosin II nanomachine mimicking the striated muscle. Nature Communications, 9(1), [3532]. https://doi.org/10.1038/s41467-018-06073-9

A myosin II nanomachine mimicking the striated muscle. / Pertici, Irene; Bongini, Lorenzo; Melli, Luca; Bianchi, Giulio; Salvi, Luca; Falorsi, Giulia; Squarci, Caterina; Bozó, Tamás; Cojoc, Dan; Kellermayer, M.; Lombardi, Vincenzo; Bianco, Pasquale.

In: Nature Communications, Vol. 9, No. 1, 3532, 01.12.2018.

Research output: Contribution to journalArticle

Pertici, I, Bongini, L, Melli, L, Bianchi, G, Salvi, L, Falorsi, G, Squarci, C, Bozó, T, Cojoc, D, Kellermayer, M, Lombardi, V & Bianco, P 2018, 'A myosin II nanomachine mimicking the striated muscle', Nature Communications, vol. 9, no. 1, 3532. https://doi.org/10.1038/s41467-018-06073-9
Pertici I, Bongini L, Melli L, Bianchi G, Salvi L, Falorsi G et al. A myosin II nanomachine mimicking the striated muscle. Nature Communications. 2018 Dec 1;9(1). 3532. https://doi.org/10.1038/s41467-018-06073-9
Pertici, Irene ; Bongini, Lorenzo ; Melli, Luca ; Bianchi, Giulio ; Salvi, Luca ; Falorsi, Giulia ; Squarci, Caterina ; Bozó, Tamás ; Cojoc, Dan ; Kellermayer, M. ; Lombardi, Vincenzo ; Bianco, Pasquale. / A myosin II nanomachine mimicking the striated muscle. In: Nature Communications. 2018 ; Vol. 9, No. 1.
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