A mutation within the catalytic domain of COT1 kinase confers changes in the presence of two COT1 isoforms and in Ser/Thr protein kinase and phosphatase activities in Neurospora crassa

Rena Gorovits, Oshrat Propheta, Mikhail Kolot, V. Dombrádi, Oded Yarden

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Neurospora crassa grows by forming spreading colonies. cot-1 belongs to a class of N. crassa colonial temperature-sensitive (cot) mutants and encodes a Ser/Thr protein kinase. We have mapped the cot-1 mutation to a single base change resulting in a His to Arg substitution at amino acid 351, which resides within the catalytic domain. Antibodies raised against COT1 detected and immunoprecipitated a predominant 73-kDa polypeptide in N. crassa extracts, whose abundance was constant under all growth conditions tested. An additional, lower MW COT1 isoform (67-kDa) present in the wild-type was not detected in cot-1 grown at the restrictive temperature. Similarly, this isoform was not detected in cot-3 or cot-5 strains, when grown at restrictive temperatures. Reduced levels of Ser/Thr kinase activity and an increase in type 1 and type 2B phosphatase (calcineurin) activities were measured in a cot-1 background. Apparent changes in the phosphorylation state of the p150(Glued) subunit of the dynactin cytoskeletal motor component (encoded by ro-3, a suppressor of cot-1) and evidence of in vitro physical interactions between COT1 and calcineurin indicate a functional linkage among COT1 kinase, type 2B phosphatase, and dynactin.

Original languageEnglish
Pages (from-to)264-274
Number of pages11
JournalFungal Genetics and Biology
Volume27
Issue number2-3
DOIs
Publication statusPublished - Jul 1999

Fingerprint

Neurospora crassa
Phosphoprotein Phosphatases
Protein Kinases
Catalytic Domain
Protein Isoforms
Phosphotransferases
Mutation
Temperature
Calcineurin
Amino Acid Substitution
Phosphoric Monoester Hydrolases
Phosphorylation
Peptides

Keywords

  • Calcineurin
  • COT1 kinase
  • Dynactin
  • Fungal growth and development
  • Neurospora crassa

ASJC Scopus subject areas

  • Genetics
  • Microbiology

Cite this

A mutation within the catalytic domain of COT1 kinase confers changes in the presence of two COT1 isoforms and in Ser/Thr protein kinase and phosphatase activities in Neurospora crassa. / Gorovits, Rena; Propheta, Oshrat; Kolot, Mikhail; Dombrádi, V.; Yarden, Oded.

In: Fungal Genetics and Biology, Vol. 27, No. 2-3, 07.1999, p. 264-274.

Research output: Contribution to journalArticle

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