A multitechnique study of Bacteriorhodopsin's photonics towards new optical devices

Marta Martin, Marie Belle Saab, Thierry Cloitre, Elias Estephan, René Legros, Frédéric J.G. Cuisinier, László Zimányi, Csilla Gergely

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Citation (Scopus)


Bacteriorhodopsin (BR) is a robust trans-membrane protein that functions as a light-driven proton pump, thus is an excellent candidate for biophotonics applications. For the development of new optical devices, the buildup of stable BR matrices has to be optimised. In this work, we present a multi- technique approach: the combination of optical waveguide lightmode spectroscopy (OWLS), atomic force microscopy (AFM) and multi-photon microscopy (MPM) aiming to analyze the optical and physico-chemical properties of BR embedded in polyelectrolyte multilayers (PEM) in its membrane bound form (purple membrane, PM), as well as solubilized BR immobilized within a photonic structure built of porous silicon (PSi). OWLS measurements revealed the possibility of incorporation of PM-BR layers into PE-multilayers. The calculated thickness and refractive index of the adsorbed layers demonstrate the successful adsorption of PM on top of the positively or negatively charged PE layers. Morphological studies by AFM proved a complete coverage of the positively charged PE layer with PM patches. As for the other model system, photonic responses of BR, after being immobilized within PSi substrates, have been evaluated using multi-photon microscopy. Fluorescence emission and second harmonic generation (SHG) of the BR-PSi system were observed at some particular pores of PSi and subsequent enhancement of the signal arising from the BR adsorbed within the pores was detected. Our results constitute the first steps of two interesting and innovative biomimetic approaches for the future design and development of BR based integrated optical devices.

Original languageEnglish
Title of host publicationBiophotonics
Subtitle of host publicationPhotonic Solutions for Better Health Care
Publication statusPublished - Jun 18 2008
EventBiophotonics: Photonic Solutions for Better Health Care - Strasbourg, France
Duration: Apr 8 2008Apr 10 2008

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
ISSN (Print)1605-7422


OtherBiophotonics: Photonic Solutions for Better Health Care


  • Atomic force microscopy
  • Bacteriorhodopsin
  • Multi-photon microscopy
  • Optical waveguide lightmode spectroscopy
  • Polyelectrolyte multilayers
  • Porous silicon microcavities
  • Protein adsorption
  • Second harmonic generation

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Radiology Nuclear Medicine and imaging

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  • Cite this

    Martin, M., Saab, M. B., Cloitre, T., Estephan, E., Legros, R., Cuisinier, F. J. G., Zimányi, L., & Gergely, C. (2008). A multitechnique study of Bacteriorhodopsin's photonics towards new optical devices. In Biophotonics: Photonic Solutions for Better Health Care [69910A] (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 6991). https://doi.org/10.1117/12.781016