A multipurpose instrument for quantitative intravital microscopy

A. Tóth, M. E. Tischler, M. Pal, A. Koller, P. C. Johnson

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

An in vivo microscope system has been developed that can measure fluorescence emission and/or light absorption at up to five wavelengths in a tissue area of 18-30 μm diam while imaging adjacent microcirculatory vessels with a video system. The system also incorporates a computer-controlled stage and data acquisition system for rapid and repeated measurements from a number of tissue sites. The tissue area monitored for fluorescence or absorption can be defined further by a confocal arrangement of the microscope optics. Tests of the system for NADH fluorescence measurements show good agreement between the fluorescence at 450 nm and NADH concentration in vitro and in skeletal muscle. The instrument can also be used simultaneously for spectrophotometric determination of O2 saturation and hematocrit in microcirculatory vessels. In vitro tests indicate suitable accuracy for such measurements. The open architecture and modular arrangement of the instrument facilitates its use for a variety of simultaneous measurements of parenchymal cell and microcirculatory function.

Original languageEnglish
Pages (from-to)296-306
Number of pages11
JournalJournal of Applied Physiology
Volume73
Issue number1
Publication statusPublished - 1992

Fingerprint

Fluorescence
NAD
Hematocrit
Information Systems
Skeletal Muscle
Intravital Microscopy
In Vitro Techniques

Keywords

  • blood flow
  • fluorescence
  • hematocrit
  • microcirculation
  • NADH
  • oxidative metabolism
  • oxygen

ASJC Scopus subject areas

  • Endocrinology
  • Physiology
  • Orthopedics and Sports Medicine
  • Physical Therapy, Sports Therapy and Rehabilitation

Cite this

Tóth, A., Tischler, M. E., Pal, M., Koller, A., & Johnson, P. C. (1992). A multipurpose instrument for quantitative intravital microscopy. Journal of Applied Physiology, 73(1), 296-306.

A multipurpose instrument for quantitative intravital microscopy. / Tóth, A.; Tischler, M. E.; Pal, M.; Koller, A.; Johnson, P. C.

In: Journal of Applied Physiology, Vol. 73, No. 1, 1992, p. 296-306.

Research output: Contribution to journalArticle

Tóth, A, Tischler, ME, Pal, M, Koller, A & Johnson, PC 1992, 'A multipurpose instrument for quantitative intravital microscopy', Journal of Applied Physiology, vol. 73, no. 1, pp. 296-306.
Tóth A, Tischler ME, Pal M, Koller A, Johnson PC. A multipurpose instrument for quantitative intravital microscopy. Journal of Applied Physiology. 1992;73(1):296-306.
Tóth, A. ; Tischler, M. E. ; Pal, M. ; Koller, A. ; Johnson, P. C. / A multipurpose instrument for quantitative intravital microscopy. In: Journal of Applied Physiology. 1992 ; Vol. 73, No. 1. pp. 296-306.
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