A multidimensional overpressured layer chromatographic method for the characterization of tetrazine libraries

Naran Gombosuren, Zoltán Novák, András Kotschy, Emil Mincsovics, Gábor Dibó

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

The recent combinatorial approach in synthetic organic chemistry started a new age in drug discovery. The generation of compound libraries in combination with high-throughput screening has become the method of choice for the production of new pharmacological leads for chemical optimization. Characterization and separation of such pool of compounds have been lagging behind the synthetic and screening methodologies. Overpressured layer chromatography (OPLC) is an instrumentalized planar liquid chromatographic technique associated with the use of optimized layers prepared from particles of narrow particle size distribution and small diameter. On one hand, uni-directional OPLC allows the simultaneous separation of large number of samples in minutes. On the other hand, two-dimensional OPLC offers multidimensional separation on a single layer. This paper shows the complete multidimensional separation of a tetrazine library prepared by parallel combinatorial synthesis. In general, this approach may become the method of choice for the characterization of compound libraries.

Original languageEnglish
Pages (from-to)239-249
Number of pages11
JournalJournal of biochemical and biophysical methods
Volume69
Issue number3
DOIs
Publication statusPublished - Jan 10 2007

Keywords

  • 2D OPLC
  • Combinatorial libraries
  • Multidimensional separation
  • Overpressured layer chromatography
  • Tetrazine

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry

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