A monoclonal antibody to Histoplasma capsulatum alters the intracellular fate of the fungus in murine macrophages

Li Shi, Priscila C. Albuquerque, Eszter Lazar-Molnar, Xintao Wang, Laura Santambrogio, A. Gácser, Joshua D. Nosanchuk

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

Monoclonal antibodies (MAbs) to a cell surface histone on Histoplasma capsulatum modify murine infection and decrease the growth of H. capsulatum within macrophages. Without the MAbs, H. capsulatum survives within macrophages by modifying the intraphagosomal environment. In the present study, we aimed to analyze the affects of a MAb on macrophage phagosomes. Using transmission electron and fluorescence microscopy, we showed that phagosome activation and maturation are significantly greater when H. capsulatum yeast are opsonized with MAb. The MAb reduced the ability of the organism to regulate the phagosomal pH. Additionally, increased antigen processing and reduced negative costimulation occur in macrophages that phagocytose yeast cells opsonized with MAb, resulting in more-efficient T-cell activation. The MAb alters the intracellular fate of H. capsulatum by affecting the ability of the fungus to regulate the milieu of the phagosome.

Original languageEnglish
Pages (from-to)1109-1117
Number of pages9
JournalEukaryotic Cell
Volume7
Issue number7
DOIs
Publication statusPublished - Jul 2008

Fingerprint

Histoplasma capsulatum
Histoplasma
monoclonal antibodies
macrophages
Fungi
phagosomes
Phagosomes
Macrophages
Monoclonal Antibodies
fungi
mice
Yeasts
yeasts
Cytophagocytosis
Antigen Presentation
fluorescence microscopy
phagocytosis
Transmission Electron Microscopy
Fluorescence Microscopy
histones

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Microbiology

Cite this

Shi, L., Albuquerque, P. C., Lazar-Molnar, E., Wang, X., Santambrogio, L., Gácser, A., & Nosanchuk, J. D. (2008). A monoclonal antibody to Histoplasma capsulatum alters the intracellular fate of the fungus in murine macrophages. Eukaryotic Cell, 7(7), 1109-1117. https://doi.org/10.1128/EC.00036-08

A monoclonal antibody to Histoplasma capsulatum alters the intracellular fate of the fungus in murine macrophages. / Shi, Li; Albuquerque, Priscila C.; Lazar-Molnar, Eszter; Wang, Xintao; Santambrogio, Laura; Gácser, A.; Nosanchuk, Joshua D.

In: Eukaryotic Cell, Vol. 7, No. 7, 07.2008, p. 1109-1117.

Research output: Contribution to journalArticle

Shi, L, Albuquerque, PC, Lazar-Molnar, E, Wang, X, Santambrogio, L, Gácser, A & Nosanchuk, JD 2008, 'A monoclonal antibody to Histoplasma capsulatum alters the intracellular fate of the fungus in murine macrophages', Eukaryotic Cell, vol. 7, no. 7, pp. 1109-1117. https://doi.org/10.1128/EC.00036-08
Shi, Li ; Albuquerque, Priscila C. ; Lazar-Molnar, Eszter ; Wang, Xintao ; Santambrogio, Laura ; Gácser, A. ; Nosanchuk, Joshua D. / A monoclonal antibody to Histoplasma capsulatum alters the intracellular fate of the fungus in murine macrophages. In: Eukaryotic Cell. 2008 ; Vol. 7, No. 7. pp. 1109-1117.
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