A monoclonal antibody to Histoplasma capsulatum alters the intracellular fate of the fungus in murine macrophages

Li Shi, Priscila C. Albuquerque, Eszter Lazar-Molnar, Xintao Wang, Laura Santambrogio, Attila Gácser, Joshua D. Nosanchuk

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29 Citations (Scopus)

Abstract

Monoclonal antibodies (MAbs) to a cell surface histone on Histoplasma capsulatum modify murine infection and decrease the growth of H. capsulatum within macrophages. Without the MAbs, H. capsulatum survives within macrophages by modifying the intraphagosomal environment. In the present study, we aimed to analyze the affects of a MAb on macrophage phagosomes. Using transmission electron and fluorescence microscopy, we showed that phagosome activation and maturation are significantly greater when H. capsulatum yeast are opsonized with MAb. The MAb reduced the ability of the organism to regulate the phagosomal pH. Additionally, increased antigen processing and reduced negative costimulation occur in macrophages that phagocytose yeast cells opsonized with MAb, resulting in more-efficient T-cell activation. The MAb alters the intracellular fate of H. capsulatum by affecting the ability of the fungus to regulate the milieu of the phagosome.

Original languageEnglish
Pages (from-to)1109-1117
Number of pages9
JournalEukaryotic Cell
Volume7
Issue number7
DOIs
Publication statusPublished - Jul 1 2008

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology

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    Shi, L., Albuquerque, P. C., Lazar-Molnar, E., Wang, X., Santambrogio, L., Gácser, A., & Nosanchuk, J. D. (2008). A monoclonal antibody to Histoplasma capsulatum alters the intracellular fate of the fungus in murine macrophages. Eukaryotic Cell, 7(7), 1109-1117. https://doi.org/10.1128/EC.00036-08