A modified semipermeable membrane technique for carbonic anhydrase histochemistry of the nervous system

A. Mihály, E. Király, A. Nógrádi, K. Benesik

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

We present a modification of Hansson's method for the demonstration of carbonic anhydrase activity. Using a semipermeable membrane together with a fluid incubation medium, frozen sections of aldehyde-fixed tissue were incubated without floating or dipping. Thin sections (thickness, 20-40 μm) were mounted on the outer surface of a tubularshaped, semipermeable cellophane dialysis membrane containing the incubation fluid. After incubation for 25-30 min at room temperature, the sections were rinsed in buffer and treated with 0.5% (NH4)2S solution. The histochemical reaction was fully inhibited by 10-4M acetazolamide.

Original languageEnglish
Pages (from-to)485-487
Number of pages3
JournalHistochemistry
Volume88
Issue number3-6
DOIs
Publication statusPublished - May 1988

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Carbonic Anhydrases
Nervous System
Cellophane
Acetazolamide
Membranes
Frozen Sections
Aldehydes
Dialysis
Buffers
Temperature

ASJC Scopus subject areas

  • Anatomy

Cite this

A modified semipermeable membrane technique for carbonic anhydrase histochemistry of the nervous system. / Mihály, A.; Király, E.; Nógrádi, A.; Benesik, K.

In: Histochemistry, Vol. 88, No. 3-6, 05.1988, p. 485-487.

Research output: Contribution to journalArticle

Mihály, A. ; Király, E. ; Nógrádi, A. ; Benesik, K. / A modified semipermeable membrane technique for carbonic anhydrase histochemistry of the nervous system. In: Histochemistry. 1988 ; Vol. 88, No. 3-6. pp. 485-487.
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