Non used interlocking bore holes of a modified UHN were penetrated with active osteogenetic tissue, after implantation in the tibial bones of the sheep. After 8 postoperative weeks the tissue originating from bone marrow and/or endost were investigated histologically and with the immunohistochemical technique. These penetrated tissue samples contain trabecular bone structures with osteolasts and osteocytes and a central bone marrow. In areas of an angiogenic desmal ossification immunoreactivities for NOS II, COX 2 and β-integrine could be demonstrated. Migrating osteoblasts contain stress fibers with β-actin immunostaining, vinculin was also found. In nerve fibers and in migrating osteoblasts an immunoreactivity for CGRP and substance P could be seen. In human tissue samples from patients with tibial fractures comparable histologically structures and specific immunoreactivities could be found. The possible role of the neuropeptides, especially of CGRP, in the osteoneogenesis during the process of fracture healing will be discussed.
|Translated title of the contribution||A modified intramedullary nail-first experimental examinations (II): Immunohistochemical investigations of tissue samples penetrating the implant bore holes|
|Number of pages||11|
|Publication status||Published - Sep 1 2006|
- Interlocking nail
- Tibia of the sheep
- Tissue penetrating of the bore holes
ASJC Scopus subject areas