A mitochondrial ferredoxin is essential for biogenesis of cellular iron- sulfur proteins

Heike Lange, Anita Kaut, G. Kispál, Roland Lill

Research output: Contribution to journalArticle

222 Citations (Scopus)

Abstract

Iron-sulfur (Fe/S) cluster-containing proteins catalyze a number of electron transfer and metabolic reactions. The components and molecular mechanisms involved in the assembly of the Fe/S clusters have been identified only partially. In eukaryotes, mitochondria have been proposed to execute a crucial task in the generation of intramitochondrial and extramitochondrial Fe/S proteins. Herein, we identify the essential ferredoxin Yah1p of Saccharomyces cerevisiae mitochondria as a central component of the Fe/S protein biosynthesis machinery. Depletion of Yah1p by regulated gene expression resulted in a 30-fold accumulation of iron within mitochondria, similar to what has been reported for other components involved in Fe/S protein biogenesis. Yah1p was shown to be required for the assembly of Fe/S proteins both inside mitochondria and in the cytosol. Apparently, at least one of the steps of Fe/S duster biogenesis within mitochondria requires reduction by ferredoxin. Our findings lend support to the idea of a primary function of mitochondria in the biosynthesis of Fe/S proteins outside the organelle. To our knowledge, Yah1p is the first member of the ferredoxin family for which a function in Fe/S cluster formation has been established. A similar role may be predicted for the bacterial homologs that are encoded within iron-sulfur cluster assembly (isc) operons of prokaryotes.

Original languageEnglish
Pages (from-to)1050-1055
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume97
Issue number3
DOIs
Publication statusPublished - 2000

Fingerprint

Iron-Sulfur Proteins
Ferredoxins
Mitochondria
Protein S
Iron
Sulfur
Protein Biosynthesis
Operon
Eukaryota
Organelles
Cytosol
Saccharomyces cerevisiae
Electrons
Gene Expression

Keywords

  • ATP-binding cassette transporter
  • Iron homeostasis

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

A mitochondrial ferredoxin is essential for biogenesis of cellular iron- sulfur proteins. / Lange, Heike; Kaut, Anita; Kispál, G.; Lill, Roland.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 97, No. 3, 2000, p. 1050-1055.

Research output: Contribution to journalArticle

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