A micromethod for the separate evaluation of phagocytosis and intracellular killing of Staaphylococcus aureus by human monocytes and granulocytes

L. Máródi, Peter C J Leijh, Ralph Van Furth

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

A micro-assay has been developed for the separate evaluation of phagocytosis and intracellular killing of Staphylococcus aureus by human monocytes and granulocytes. the minimal number of phagocytes required for the investigation of these functional activities of phagocytic cells has been established by performing phagocytosis and intracellular killing experiments at various cell concentrations, bacteria-to-cell ratios, and volumes. The results of these experiments revealed that phagocytois can be measured in a reliable way, at bacteria-to-cell ratios of 5:1 and 1: (cell concentration 5×106/ml), in a suspension of 200 μl. The rate of tracellular killing by monocytes and granulocytes can also be measured with a total phagocytic suspension of 200 μl and a cell concentration of 5×106/ml. From these results it can be concluded that for an independent determination of the phagocytosis and intracellular killing of micro-organisms, 400 μl of 5×106 phagocytes/ml is required, i.e., a total of 2×106 phagocytes. This number of granulocytes can be obtained from 1-2 ml of blood; for monocytes 4-8 ml of blood is required.

Original languageEnglish
Pages (from-to)353-361
Number of pages9
JournalJournal of Immunological Methods
Volume57
Issue number1-3
DOIs
Publication statusPublished - Feb 25 1983

Fingerprint

Phagocytes
Phagocytosis
Granulocytes
Monocytes
Suspensions
Bacteria
Cell Size
Staphylococcus aureus

Keywords

  • granulocytes
  • intracellular killing
  • monocytes
  • phagocytosis

ASJC Scopus subject areas

  • Biotechnology
  • Immunology

Cite this

A micromethod for the separate evaluation of phagocytosis and intracellular killing of Staaphylococcus aureus by human monocytes and granulocytes. / Máródi, L.; Leijh, Peter C J; Van Furth, Ralph.

In: Journal of Immunological Methods, Vol. 57, No. 1-3, 25.02.1983, p. 353-361.

Research output: Contribution to journalArticle

@article{1afb8233fb614420874abe5d347d32c3,
title = "A micromethod for the separate evaluation of phagocytosis and intracellular killing of Staaphylococcus aureus by human monocytes and granulocytes",
abstract = "A micro-assay has been developed for the separate evaluation of phagocytosis and intracellular killing of Staphylococcus aureus by human monocytes and granulocytes. the minimal number of phagocytes required for the investigation of these functional activities of phagocytic cells has been established by performing phagocytosis and intracellular killing experiments at various cell concentrations, bacteria-to-cell ratios, and volumes. The results of these experiments revealed that phagocytois can be measured in a reliable way, at bacteria-to-cell ratios of 5:1 and 1: (cell concentration 5×106/ml), in a suspension of 200 μl. The rate of tracellular killing by monocytes and granulocytes can also be measured with a total phagocytic suspension of 200 μl and a cell concentration of 5×106/ml. From these results it can be concluded that for an independent determination of the phagocytosis and intracellular killing of micro-organisms, 400 μl of 5×106 phagocytes/ml is required, i.e., a total of 2×106 phagocytes. This number of granulocytes can be obtained from 1-2 ml of blood; for monocytes 4-8 ml of blood is required.",
keywords = "granulocytes, intracellular killing, monocytes, phagocytosis",
author = "L. M{\'a}r{\'o}di and Leijh, {Peter C J} and {Van Furth}, Ralph",
year = "1983",
month = "2",
day = "25",
doi = "10.1016/0022-1759(83)90095-9",
language = "English",
volume = "57",
pages = "353--361",
journal = "Journal of Immunological Methods",
issn = "0022-1759",
publisher = "Elsevier",
number = "1-3",

}

TY - JOUR

T1 - A micromethod for the separate evaluation of phagocytosis and intracellular killing of Staaphylococcus aureus by human monocytes and granulocytes

AU - Máródi, L.

AU - Leijh, Peter C J

AU - Van Furth, Ralph

PY - 1983/2/25

Y1 - 1983/2/25

N2 - A micro-assay has been developed for the separate evaluation of phagocytosis and intracellular killing of Staphylococcus aureus by human monocytes and granulocytes. the minimal number of phagocytes required for the investigation of these functional activities of phagocytic cells has been established by performing phagocytosis and intracellular killing experiments at various cell concentrations, bacteria-to-cell ratios, and volumes. The results of these experiments revealed that phagocytois can be measured in a reliable way, at bacteria-to-cell ratios of 5:1 and 1: (cell concentration 5×106/ml), in a suspension of 200 μl. The rate of tracellular killing by monocytes and granulocytes can also be measured with a total phagocytic suspension of 200 μl and a cell concentration of 5×106/ml. From these results it can be concluded that for an independent determination of the phagocytosis and intracellular killing of micro-organisms, 400 μl of 5×106 phagocytes/ml is required, i.e., a total of 2×106 phagocytes. This number of granulocytes can be obtained from 1-2 ml of blood; for monocytes 4-8 ml of blood is required.

AB - A micro-assay has been developed for the separate evaluation of phagocytosis and intracellular killing of Staphylococcus aureus by human monocytes and granulocytes. the minimal number of phagocytes required for the investigation of these functional activities of phagocytic cells has been established by performing phagocytosis and intracellular killing experiments at various cell concentrations, bacteria-to-cell ratios, and volumes. The results of these experiments revealed that phagocytois can be measured in a reliable way, at bacteria-to-cell ratios of 5:1 and 1: (cell concentration 5×106/ml), in a suspension of 200 μl. The rate of tracellular killing by monocytes and granulocytes can also be measured with a total phagocytic suspension of 200 μl and a cell concentration of 5×106/ml. From these results it can be concluded that for an independent determination of the phagocytosis and intracellular killing of micro-organisms, 400 μl of 5×106 phagocytes/ml is required, i.e., a total of 2×106 phagocytes. This number of granulocytes can be obtained from 1-2 ml of blood; for monocytes 4-8 ml of blood is required.

KW - granulocytes

KW - intracellular killing

KW - monocytes

KW - phagocytosis

UR - http://www.scopus.com/inward/record.url?scp=0020630725&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0020630725&partnerID=8YFLogxK

U2 - 10.1016/0022-1759(83)90095-9

DO - 10.1016/0022-1759(83)90095-9

M3 - Article

VL - 57

SP - 353

EP - 361

JO - Journal of Immunological Methods

JF - Journal of Immunological Methods

SN - 0022-1759

IS - 1-3

ER -