A mechanistic view of lipid membrane disrupting effect of PAMAM dendrimers

Szilvia Berényi, Judith Mihály, András Wacha, Orsolya Toke, Attila Bóta

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

The effect of 5th generation polyamidoamine (PAMAM G5) dendrimers on multilamellar dipalmitoylphosphocholine (DPPC) vesicles was investigated. PAMAM was added in two different concentrations to the lipids (10-3 and 10-2 dendrimer/lipid molar ratios). The thermal behavior of the evolved systems was characterized by DSC; while the structure and the morphology were investigated with small- and wide-angel X-ray scattering (SWAXS), freeze-fracture electron microscopy (FFTEM) and phosphorus-31 nuclear magnetic resonance (31P NMR) spectroscopy, respectively. IR spectroscopy was used to study the molecular interactions between PAMAM and DPPC. The obtained results show that the dendrimers added in 10-3 molar ratio to the lipids generate minor perturbations in the multilamellar structure and thermal character of liposomes, while added in 10-2 molar ratio dendrimers cause major disturbance in the vesicular system. The terminal amino groups of the dendrimers are in strong interaction with the phosphate headgroups and through this binding dendrimers disrupt the regular multilamellar structure of DPPC. Besides highly swollen, fragmented bilayers, small vesicles are formed.

Original languageEnglish
Pages (from-to)164-171
Number of pages8
JournalColloids and Surfaces B: Biointerfaces
Volume118
DOIs
Publication statusPublished - Jun 1 2014

Keywords

  • DPPC
  • Dendrimer
  • Infrared spectroscopy
  • Model membrane
  • SAXS

ASJC Scopus subject areas

  • Biotechnology
  • Surfaces and Interfaces
  • Physical and Theoretical Chemistry
  • Colloid and Surface Chemistry

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