A mass spectrometry based functional assay for the quantitative assessment of ABC transporter activity

Maria Katona, Katalin Kiss, Vilmos Angyal, Nóra Kucsma, Balázs Sarkadi, Zoltán Takáts, Gergely Szakács

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

ATP-Binding Cassette (ABC) transporters are highly expressed in pharmacological barriers limiting the access of drugs to their targets. Since characterization of a compound as a transporter substrate or inhibitor bears significant consequences in drug development, there is a great need for reliable tools that enable the rapid analysis of the transport susceptibility of drugs. Here we describe a simple but very efficient high-performance liquid chromatography/mass spectrometry (HPLC/MS) assay for measuring the ABC transporter-dependent vesicular transport of compounds. In addition, we provide evidence that the requirement for sample preparation can be minimized using desorption electrospray ionization (DESI)-MS, paving the way for a direct, high-throughput investigation of drug-transporter interactions.

Original languageEnglish
Pages (from-to)3372-3376
Number of pages5
JournalRapid Communications in Mass Spectrometry
Volume23
Issue number21
DOIs
Publication statusPublished - Nov 15 2009

ASJC Scopus subject areas

  • Analytical Chemistry
  • Spectroscopy
  • Organic Chemistry

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