A mannose-binding receptor is expressed on human keratinocytes and mediates killing of Candida albicans

Gyözö Szolnoky, Z. Bata-Csörgő, Anna Sz Kenderessy, M. Kiss, Andor Pivarcsi, Zoltán Novák, K. Nagy, Günther Michel, Thomas Ruzicka, L. Máródi, A. Dobozy, L. Kemény

Research output: Contribution to journalArticle

62 Citations (Scopus)

Abstract

Human keratinocytes are known to kill Candida albicans in vitro, but the mechanism of killing is not yet understood. Here, we demonstrate that spontaneous, ultraviolet-B-light-induced, α-melanocyte-stimulating-hormone-induced, and interleukin-8-induced Candida killing by keratinocytes can be inhibited with mannan and mannosylated bovine serum albumin (Man-BSA). A polyclonal goat serum raised against the human macrophage mannose receptor stained suprabasal keratinocytes, but no staining was observed on keratinocytes with a monoclonal anti-body (mAb15) specific for the human macrophage mannose receptor. Mannose-afinity chromatography of keratinocyte extract isolated a 200 kDa protein, and on the Western blot the goat antiserum reacted with a 200 kDa protein. In radioligand binding studies, the binding of 125I-Man-BSA to human keratinocytes was inhibited by mannan in a concentration-dependent manner. Analysis of the binding revealed a single class keratinocyte mannose receptor with a KD of 1.4 10-8 M and a Bmax of 1 104 binding sites per cell. The binding of 125I-Man-BSA to keratinocytes proved to be time-dependent, acid-precipitable, and Ca2+- and trypsin-sensitive. After trypsinization the receptors underwent a rapid recovery at 37°C. These results demonstrate the presence of mannose receptor on human keratinocytes, and its active involvement in the killing of Candida albicans.

Original languageEnglish
Pages (from-to)205-213
Number of pages9
JournalJournal of Investigative Dermatology
Volume117
Issue number2
DOIs
Publication statusPublished - 2001

Fingerprint

Candida
Mannose
Candida albicans
Keratinocytes
Mannans
Macrophages
Goats
Melanocyte-Stimulating Hormones
mannose receptor
Ultraviolet Rays
Chromatography
Interleukin-8
Trypsin
Immune Sera
Proteins
Western Blotting
Binding Sites
Staining and Labeling
Recovery
Acids

Keywords

  • Antimicrobial activity
  • Candida infection
  • Epidermis
  • Skin

ASJC Scopus subject areas

  • Dermatology

Cite this

A mannose-binding receptor is expressed on human keratinocytes and mediates killing of Candida albicans. / Szolnoky, Gyözö; Bata-Csörgő, Z.; Kenderessy, Anna Sz; Kiss, M.; Pivarcsi, Andor; Novák, Zoltán; Nagy, K.; Michel, Günther; Ruzicka, Thomas; Máródi, L.; Dobozy, A.; Kemény, L.

In: Journal of Investigative Dermatology, Vol. 117, No. 2, 2001, p. 205-213.

Research output: Contribution to journalArticle

Szolnoky, Gyözö ; Bata-Csörgő, Z. ; Kenderessy, Anna Sz ; Kiss, M. ; Pivarcsi, Andor ; Novák, Zoltán ; Nagy, K. ; Michel, Günther ; Ruzicka, Thomas ; Máródi, L. ; Dobozy, A. ; Kemény, L. / A mannose-binding receptor is expressed on human keratinocytes and mediates killing of Candida albicans. In: Journal of Investigative Dermatology. 2001 ; Vol. 117, No. 2. pp. 205-213.
@article{de2e29279435435ba93058392590720f,
title = "A mannose-binding receptor is expressed on human keratinocytes and mediates killing of Candida albicans",
abstract = "Human keratinocytes are known to kill Candida albicans in vitro, but the mechanism of killing is not yet understood. Here, we demonstrate that spontaneous, ultraviolet-B-light-induced, α-melanocyte-stimulating-hormone-induced, and interleukin-8-induced Candida killing by keratinocytes can be inhibited with mannan and mannosylated bovine serum albumin (Man-BSA). A polyclonal goat serum raised against the human macrophage mannose receptor stained suprabasal keratinocytes, but no staining was observed on keratinocytes with a monoclonal anti-body (mAb15) specific for the human macrophage mannose receptor. Mannose-afinity chromatography of keratinocyte extract isolated a 200 kDa protein, and on the Western blot the goat antiserum reacted with a 200 kDa protein. In radioligand binding studies, the binding of 125I-Man-BSA to human keratinocytes was inhibited by mannan in a concentration-dependent manner. Analysis of the binding revealed a single class keratinocyte mannose receptor with a KD of 1.4 10-8 M and a Bmax of 1 104 binding sites per cell. The binding of 125I-Man-BSA to keratinocytes proved to be time-dependent, acid-precipitable, and Ca2+- and trypsin-sensitive. After trypsinization the receptors underwent a rapid recovery at 37°C. These results demonstrate the presence of mannose receptor on human keratinocytes, and its active involvement in the killing of Candida albicans.",
keywords = "Antimicrobial activity, Candida infection, Epidermis, Skin",
author = "Gy{\"o}z{\"o} Szolnoky and Z. Bata-Cs{\"o}rgő and Kenderessy, {Anna Sz} and M. Kiss and Andor Pivarcsi and Zolt{\'a}n Nov{\'a}k and K. Nagy and G{\"u}nther Michel and Thomas Ruzicka and L. M{\'a}r{\'o}di and A. Dobozy and L. Kem{\'e}ny",
year = "2001",
doi = "10.1046/j.1523-1747.2001.14071.x",
language = "English",
volume = "117",
pages = "205--213",
journal = "Journal of Investigative Dermatology",
issn = "0022-202X",
publisher = "Nature Publishing Group",
number = "2",

}

TY - JOUR

T1 - A mannose-binding receptor is expressed on human keratinocytes and mediates killing of Candida albicans

AU - Szolnoky, Gyözö

AU - Bata-Csörgő, Z.

AU - Kenderessy, Anna Sz

AU - Kiss, M.

AU - Pivarcsi, Andor

AU - Novák, Zoltán

AU - Nagy, K.

AU - Michel, Günther

AU - Ruzicka, Thomas

AU - Máródi, L.

AU - Dobozy, A.

AU - Kemény, L.

PY - 2001

Y1 - 2001

N2 - Human keratinocytes are known to kill Candida albicans in vitro, but the mechanism of killing is not yet understood. Here, we demonstrate that spontaneous, ultraviolet-B-light-induced, α-melanocyte-stimulating-hormone-induced, and interleukin-8-induced Candida killing by keratinocytes can be inhibited with mannan and mannosylated bovine serum albumin (Man-BSA). A polyclonal goat serum raised against the human macrophage mannose receptor stained suprabasal keratinocytes, but no staining was observed on keratinocytes with a monoclonal anti-body (mAb15) specific for the human macrophage mannose receptor. Mannose-afinity chromatography of keratinocyte extract isolated a 200 kDa protein, and on the Western blot the goat antiserum reacted with a 200 kDa protein. In radioligand binding studies, the binding of 125I-Man-BSA to human keratinocytes was inhibited by mannan in a concentration-dependent manner. Analysis of the binding revealed a single class keratinocyte mannose receptor with a KD of 1.4 10-8 M and a Bmax of 1 104 binding sites per cell. The binding of 125I-Man-BSA to keratinocytes proved to be time-dependent, acid-precipitable, and Ca2+- and trypsin-sensitive. After trypsinization the receptors underwent a rapid recovery at 37°C. These results demonstrate the presence of mannose receptor on human keratinocytes, and its active involvement in the killing of Candida albicans.

AB - Human keratinocytes are known to kill Candida albicans in vitro, but the mechanism of killing is not yet understood. Here, we demonstrate that spontaneous, ultraviolet-B-light-induced, α-melanocyte-stimulating-hormone-induced, and interleukin-8-induced Candida killing by keratinocytes can be inhibited with mannan and mannosylated bovine serum albumin (Man-BSA). A polyclonal goat serum raised against the human macrophage mannose receptor stained suprabasal keratinocytes, but no staining was observed on keratinocytes with a monoclonal anti-body (mAb15) specific for the human macrophage mannose receptor. Mannose-afinity chromatography of keratinocyte extract isolated a 200 kDa protein, and on the Western blot the goat antiserum reacted with a 200 kDa protein. In radioligand binding studies, the binding of 125I-Man-BSA to human keratinocytes was inhibited by mannan in a concentration-dependent manner. Analysis of the binding revealed a single class keratinocyte mannose receptor with a KD of 1.4 10-8 M and a Bmax of 1 104 binding sites per cell. The binding of 125I-Man-BSA to keratinocytes proved to be time-dependent, acid-precipitable, and Ca2+- and trypsin-sensitive. After trypsinization the receptors underwent a rapid recovery at 37°C. These results demonstrate the presence of mannose receptor on human keratinocytes, and its active involvement in the killing of Candida albicans.

KW - Antimicrobial activity

KW - Candida infection

KW - Epidermis

KW - Skin

UR - http://www.scopus.com/inward/record.url?scp=18344371425&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=18344371425&partnerID=8YFLogxK

U2 - 10.1046/j.1523-1747.2001.14071.x

DO - 10.1046/j.1523-1747.2001.14071.x

M3 - Article

VL - 117

SP - 205

EP - 213

JO - Journal of Investigative Dermatology

JF - Journal of Investigative Dermatology

SN - 0022-202X

IS - 2

ER -