A light stabilizer Tinuvin 770-induced toxic injury of adult rat cardiac myocytes

P. Sótónyi, E. Keller, J. Járay, B. Nemes, T. Benkõ, A. Kovács, A. Tolokán, I. Rajs

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Tinuvin 770/bis(2,2,6,6-tetramethyl-4-piperidinyl)sebacate is a worldwide used light stabilizer for plastic materials like polyolefins. Tinuvin 770 is a biologically active component of polypropylene tubes. Glossmann and his study group managed to extract this compound by aqueous or organic solvents from laboratory plastic tubes, and propose that Tinuvin 770 is a potent blocker of L-type Ca2+-channel through the phenylalkylamine and benzothiazepine-selective drug binding domains of the α1 subunit of the receptor [Proc. Natl. Acad. Sci. U.S.A. 90 (1993) 9523].We examined the direct morphological effect of Tinuvin 770 in give 25nmol, 0, 30, 60, 120 minute exposure time in isolated cardiomyocytes from adult rats. Incubation of myocytes with Tinuvin resulted in a progressive decline of rod-shaped and viable cells. It was accompanied by an increase in number of hypercontracted myocytes with microbleb formation compared to control and depletion of ATP level.In summary, our results demonstrate that plasma membrane damage and hypercontraction are manifestations of Tinuvin-induced injury of isolated cardiomyocytes.

Original languageEnglish
Pages (from-to)322-327
Number of pages6
JournalForensic Science International
Volume119
Issue number3
DOIs
Publication statusPublished - Jul 15 2001

Fingerprint

Poisons
Cardiac Myocytes
Rats
Light
Wounds and Injuries
Muscle Cells
Plastics
Polypropylenes
Cell membranes
Organic solvents
Adenosine Triphosphate
Cell Membrane
Tinuvin 770
Pharmaceutical Preparations
tinuvin

Keywords

  • BTMPS
  • Electron microscopy
  • Isolated cardiomyocytes
  • Microbleb
  • Tinuvin 770
  • Toxic cell injury

ASJC Scopus subject areas

  • Genetics
  • Analytical Chemistry
  • Pathology and Forensic Medicine
  • Psychiatry and Mental health

Cite this

A light stabilizer Tinuvin 770-induced toxic injury of adult rat cardiac myocytes. / Sótónyi, P.; Keller, E.; Járay, J.; Nemes, B.; Benkõ, T.; Kovács, A.; Tolokán, A.; Rajs, I.

In: Forensic Science International, Vol. 119, No. 3, 15.07.2001, p. 322-327.

Research output: Contribution to journalArticle

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